Muricauda hymeniacidonis sp. nov., isolated from sponge of Hymeniacidon sinapium

• Published in: International journal of systematic and evolutionary microbiology Vol. 69; no. 12; pp. 3800 - 3805
• Main Author: Park, Jin-Sook
• Format: Journal Article
• Language: English
• Published: England 01.12.2019
• Subjects: Animals; Bacterial Typing Techniques; Base Composition; DNA, Bacterial - genetics; Fatty Acids - chemistry; Flavobacteriaceae - classification; Flavobacteriaceae - isolation & purification; Phospholipids - chemistry; Phylogeny; Pigmentation; Porifera - microbiology; Republic of Korea; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; Republic of Korea; sponge; Muricauda hymeniacidonis; 16S rRNA gene sequence; polyphasic taxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.003683

A Gram-stain-negative, rod-shaped, non-motile, aerobic, orange-pigmented, bacterium (designated 176CP4-71 ) was isolated from a sponge sample of from Cheongpo beach, Taean district, in the Republic of Korea. On the basis of 16S rRNA gene sequencing, strain 176CP4-71 clustered with species of the genus and appeared to be closely related to LMG 26637 (95.4 %), JCM 18450 (94.7 %), KACC 16195 (94.7 %) and KCTC 22339 (94.2 %). The average nucleotide identity values between strain 176CP4-71 and the closely related strains were 70.7-73.7 %, indicating that 176CP4-71 is a novel species of the genus . Growth occurs at 10-35 °C on marine agar medium in the presence of 1-8 % NaCl (w/v) and at pH 6.0-8.5. The DNA G+C content of the genomic DNA was 41.6 mol %, and menaquinone with six isoprene units (MK-6) was the major respiratory quinone. The major cellular fatty acids (>5 %) were C iso, C iso G, C iso 3OH and C ω6 and/or C ω7 (summed feature 3). The polar lipids consisted of phosphatidylethanolamine, three unidentified aminophospholipids, an unidentified phospholipid and unidentified lipids. Physiological and biochemical characteristics indicated that strain 176CP4-71 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 176CP4-71 (=KACC 19889 =LMG 31033 ).