Mesorhizobium calcicola sp. nov., Mesorhizobium waitakense sp. nov., Mesorhizobium sophorae sp. nov., Mesorhizobium newzealandense sp. nov. and Mesorhizobium kowhaii sp. nov. isolated from Sophora root nodules
In total, 31 strains of Gram-stain-negative, rod-shaped bacteria were isolated from root nodules and authenticated as rhizobia on this host. Based on 16S rRNA gene phylogeny, they were shown to belong to the genus , with the representative strains ICMP 19560 , ICMP 19523 , ICMP 19535 , ICMP 19545 an...
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Published in | International journal of systematic and evolutionary microbiology Vol. 66; no. 2; pp. 786 - 795 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
England
01.02.2016
|
Online Access | Get full text |
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Summary: | In total, 31 strains of Gram-stain-negative, rod-shaped bacteria were isolated from
root nodules and authenticated as rhizobia on this host. Based on 16S rRNA gene phylogeny, they were shown to belong to the genus
, with the representative strains ICMP 19560
, ICMP 19523
, ICMP 19535
, ICMP 19545
and ICMP 19512
being related most closely to
SCAU7
(99.9-99.6 % similarity),
ICMP 19515
(99.7-99.6 %) and
UMP-CA7
(99.7-99.5 %). Additionally, the novel strains formed distinct groups based on housekeeping gene sequence analysis and were closely related to
ICMP 19557
(93.5-94.9, 92.5-95.6 and 94.2-96.0 %),
ICMP 19515
(93.1-97.7, 93.5-95.4 and 94.8-96.8 %) and
UMP-CA7
(93.2-97.2, 94.6-96.8 and 95.5-97.3 %) for
,
and
, respectively. Chemotaxonomic data supported the assignment of the strains to the genus
, and DNA-DNA hybridizations, matrix-assisted laser desorption/ionization time-of-flight MS analysis, enterobacterial repetitive intergenic consensus PCR, physiological and biochemical tests allowed the genotypic and phenotypic differentiation from their nearest neighbouring species. Therefore, these strains represent five novel species for which the names
sp. nov. (type strain ICMP 19560
= LMG 28224
= HAMBI 3609
),
sp. nov. (type strain ICMP 19523
= LMG 28227
= HAMBI 3605
),
sp. nov. (type strain ICMP 19535
= LMG 28223
= HAMBI 3606
),
sp. nov. (type strain ICMP 19545
= LMG 28226
= HAMBI 3607
) and
sp. nov. (type strain ICMP 19512
= LMG 28222
= HAMBI 3603
) are proposed. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1466-5026 1466-5034 |
DOI: | 10.1099/ijsem.0.000796 |