Small molecule fluorescent probes of protein vicinal dithiols

• Published in: Chinese chemical letters Vol. 30; no. 10; pp. 1704 - 1716
• Main Authors: Hu, Guodong, Jia, Huiyi, Zhao, Lanning, Cho, Dong-Hyung, Fang, Jianguo
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.10.2019; State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China%School of Life Sciences, Kyungpook National University, Daegu 41566, Republic of Korea
• Subjects: Arsenical probe; Fluorescent probe; Protein vicinal dithiols; Reaction-based probe; Thiol redox; Reaction-based probe; Protein vicinal dithiols; Thiol redox; Arsenical probe; Fluorescent probe
• ISSN: 1001-8417; 1878-5964
• DOI: 10.1016/j.cclet.2019.06.039

Small molecule fluorescent probes that recognize and label protein vicinal dithiols have been summarized according to their different acceptor sites. This review will provide the purposeful design strategy of novel probes for detecting vicinal dithiols. [Display omitted] The vicinal dithiol motif is widely present in proteins, and is critical for proteins’ structures and functions. In recent years, a variety of fluorescent probes with high specificity and outstanding optical properties for sensing protein vicinal dithiols have been developed. In this review, we summarized the fluorescent probes of protein vicinal dithiols in literature. These probes are classified into four types based on their acceptor sites, i.e., biarsenical probes, monoarsenical probes, dimaleimide probes and diacrylate probes. Through analyzing the properties of different probes, we expect that this review would help readers further understand the structural factors of these probes and provide the design strategy for novel fluorescent probes with improved properties.