Determination of encainide and its metabolites by high-performance liquid chromatography
Encainide (ENC) and its metabolites O-demethylencainide (ODE), 3-methoxy-O-demethylencainide (MODE), N-demethylencainide (NDE) and bis-N,O-demethylencainide (NODE) have been measured by two HPLC procedures. The method of Mayol using a mu Porasil column with ethanol-water-methanesulphonic acid as mob...
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Published in | Journal of pharmaceutical and biomedical analysis Vol. 7; no. 3; p. 355 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
England
1989
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Subjects | |
Online Access | Get more information |
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Summary: | Encainide (ENC) and its metabolites O-demethylencainide (ODE), 3-methoxy-O-demethylencainide (MODE), N-demethylencainide (NDE) and bis-N,O-demethylencainide (NODE) have been measured by two HPLC procedures. The method of Mayol using a mu Porasil column with ethanol-water-methanesulphonic acid as mobile phase was not able to separately measure NODE and NDE in plasma. A new method is described using a mu Bondapak Phenyl column with acetonitrile-phosphate buffer (0.05 M, pH 7.5) that yields satisfactory separation of ENC and its metabolites. NODE was not identified as a metabolite in 23 patients analysed. |
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ISSN: | 0731-7085 |
DOI: | 10.1016/0731-7085(89)80102-5 |