Determination of encainide and its metabolites by high-performance liquid chromatography

Encainide (ENC) and its metabolites O-demethylencainide (ODE), 3-methoxy-O-demethylencainide (MODE), N-demethylencainide (NDE) and bis-N,O-demethylencainide (NODE) have been measured by two HPLC procedures. The method of Mayol using a mu Porasil column with ethanol-water-methanesulphonic acid as mob...

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Bibliographic Details
Published inJournal of pharmaceutical and biomedical analysis Vol. 7; no. 3; p. 355
Main Authors Selinger, K, Crawhall, J C
Format Journal Article
LanguageEnglish
Published England 1989
Subjects
Anilides - analysis
Anilides - blood
Anilides - metabolism
Anti-Arrhythmia Agents - analysis
Anti-Arrhythmia Agents - blood
Anti-Arrhythmia Agents - metabolism
Biotransformation
Chromatography, High Pressure Liquid
Dealkylation
Encainide
Humans
Spectrophotometry, Ultraviolet
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Summary:Encainide (ENC) and its metabolites O-demethylencainide (ODE), 3-methoxy-O-demethylencainide (MODE), N-demethylencainide (NDE) and bis-N,O-demethylencainide (NODE) have been measured by two HPLC procedures. The method of Mayol using a mu Porasil column with ethanol-water-methanesulphonic acid as mobile phase was not able to separately measure NODE and NDE in plasma. A new method is described using a mu Bondapak Phenyl column with acetonitrile-phosphate buffer (0.05 M, pH 7.5) that yields satisfactory separation of ENC and its metabolites. NODE was not identified as a metabolite in 23 patients analysed.
ISSN:0731-7085
DOI:10.1016/0731-7085(89)80102-5