Immobilized Yeast Membranes as Biocatalysts for Sucrose Inversion

Yeast membranes were obtained by autolysis of various strains with relatively high invertase activity. Heterogeneous biocatalysts for sucrose inversion were made of the yeast membranes and granulated carbon-containing supports made of common natural materials: expanded clay aggregate (ECA), sapropel...

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Bibliographic Details
Published inApplied biochemistry and microbiology Vol. 41; no. 4; pp. 399 - 403
Main Authors Kovalenko, G A, Perminova, LV, Plaksin, G V, Komova, O V, Chuenko, T V, Rudina, NA
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Nature B.V 01.07.2005
Subjects
Adsorption
Autolysis
beta -Fructofuranosidase
biocatalysts
Carbon
Clays
Inversion
Lignin
Sucrose
Yeast
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Summary:Yeast membranes were obtained by autolysis of various strains with relatively high invertase activity. Heterogeneous biocatalysts for sucrose inversion were made of the yeast membranes and granulated carbon-containing supports made of common natural materials: expanded clay aggregate (ECA), sapropel, and lignin. The properties of these biocatalysts were studied. It was shown that the biocatalyst activity and stability of the immobilized yeast membranes increased with reference to the initial ECA, independent of the morphology of the carbon layer synthesized on the support surface. Heterogeneous biocatalysts prepared by adsorption of yeast membranes on sapropel had the greatest activity and stability, whereas lignin-based biocatalysts were relatively unstable.
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ISSN:0003-6838
1608-3024
DOI:10.1007/s10438-005-0068-7