Microbulbifer harenosus sp. nov., an alginate-degrading bacterium isolated from coastal sand

• Published in: International journal of systematic and evolutionary microbiology Vol. 70; no. 3; pp. 1639 - 1643
• Main Authors: Huang, Huiqin, Mo, Kunlian, Hu, Yonghua, Liu, Min, Zhu, Jun, Zou, Xiaoxiao, Bao, Shixiang
• Format: Journal Article
• Language: English
• Published: England 01.03.2020
• Subjects: Alginates - metabolism; Alteromonadaceae - classification; Alteromonadaceae - isolation & purification; Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial - genetics; Fatty Acids - chemistry; Nucleic Acid Hybridization; Phylogeny; RNA, Ribosomal, 16S - genetics; Sand - microbiology; Sequence Analysis, DNA; Ubiquinone - chemistry; China; alginate-degrading; Microbulbifer harenosus sp. nov; 16S rRNA gene; polyphasic taxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.003945

A Gram-stain-negative, aerobic, rod-shaped bacterium with peritrichous flagella, designated strain HB161719 , was isolated from coastal sand collected from Tanmen Port in Hainan, PR China. The isolate was found to grow with 2-11 % (w/v) NaCl, at 15-45 °C and pH 6.0-10.0, with an optima of 2-3 % NaCl, 37 °C and pH 7.0, respectively. Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C and summed features 3, 8 and 9 were the major cellular fatty acids. The G+C content of the genomic DNA was 58.2 mol%. Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus , sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of ABABA23 , SPO729 , CC-LN1-12 and GY2 , respectively. Low DNA-DNA hybridization values showed that it formed a distinct genomic species. The combined phenotypic and molecular features supported that strain HB161719 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HB161719 (=CGMCC 1.13584 =JCM 32688 ).