Senegalimassilia faecalis sp. nov., an anaerobic actinobacterium isolated from human faeces, and emended description of the genus Senegalimassilia

• Published in: International journal of systematic and evolutionary microbiology Vol. 70; no. 3; pp. 1684 - 1690
• Main Authors: Han, Kook-Il, Kim, Ji-Sun, Lee, Keun Chul, Eom, Mi Kyung, Suh, Min Kuk, Kim, Han Sol, Park, Seung-Hwan, Lee, Ju Huck, Kang, Se Won, Park, Jam-Eon, Oh, Byeong Seob, Yu, Seung Yeob, Choi, Seung-Hyeon, Lee, Dong Ho, Yoon, Hyuk, Kim, Byung-Yong, Lee, Je Hee, Lee, Jung-Sook
• Format: Journal Article
• Language: English
• Published: England 01.03.2020
• Subjects: Actinobacteria - classification; Actinobacteria - isolation & purification; Anaerobiosis; Bacterial Typing Techniques; Base Composition; DNA, Bacterial - genetics; Fatty Acids - chemistry; Feces - microbiology; Glycolipids - chemistry; Humans; Phospholipids - chemistry; Phylogeny; Republic of Korea; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Republic of Korea; actinobacteria; Senegalimassilia faecalis; human faeces; taxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.003958

A novel actinobacterial strain, designated KGMB04484 , was isolated from healthy human faeces sampled in the Republic of Korea. Cells of strain KGMB04484 were strictly anaerobic, Gram-stain-positive, catalase-positive, oxidase-negative, non-motile coccobacilli and formed tiny colonies on Columbia agar with 5 % horse blood. On the basis of 16S rRNA gene sequence similarity, strain KGMB04484 was affiliated with the genus in the family and its closest relative was JC110 (96.28 % sequence similarity). The DNA G+C content of strain KGMB04484 was 61.2 mol%. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid, an unidentified aminolipid and three unidentified glycolipids. The predominant cellular fatty acids (>10 %) of strain KGMB04484 were C , C and C dimethyl acetal. Based on its phylogenetic, physiological and chemotaxonomic characteristics, strain KGMB04484 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is KGMB04484 (=KCTC 15721 =CCUG 72347 ).