Chemiluminescence monitoring of hemolysis by lysophospholipids

Hemolysis by 1-myristoyl- and 1-palmitoyl- sn-glycero-3-phosphocholine was monitored by the chemiluminescence of unsubstituted and long alkyl chain isoluminols. The times required for the appearance of chemiluminescence signals versus lysophospholipid concentrations show an abrupt drop at the critic...

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Bibliographic Details
Published inJournal of photochemistry and photobiology. A, Chemistry. Vol. 91; no. 2; pp. 121 - 124
Main Authors Varveri, F.S., Mavrommati, M.V., Nikokavouras, J.
Format Journal Article
LanguageEnglish
Published Lausanne Elsevier B.V 25.10.1995
Elsevier Science
Subjects
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chemiluminescence
Chemistry
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General and physical chemistry
Glycerolipids, phospholipids
Hemolysis
Isoslumino
Lipids
Lysophospholipids
Other biological molecules
Hemolysis
Lysophospholipids
Isoslumino
Chemiluminescence
Vertebrata
Venom
Red blood cell
Lysophospholipid
Reptilia
Luminescence decay
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Summary:Hemolysis by 1-myristoyl- and 1-palmitoyl- sn-glycero-3-phosphocholine was monitored by the chemiluminescence of unsubstituted and long alkyl chain isoluminols. The times required for the appearance of chemiluminescence signals versus lysophospholipid concentrations show an abrupt drop at the critical micelle concentrations of the lysophospholipids, offering evidence that lysophospholipids attack the red cell membrane as micelles. Light integrals and temperature effects are also discussed.
ISSN:1010-6030
1873-2666
DOI:10.1016/1010-6030(95)04106-P