Sporofaciens musculi gen. nov., sp. nov., a novel bacterium isolated from the caecum of an obese mouse

• Published in: International journal of systematic and evolutionary microbiology Vol. 71; no. 3
• Main Authors: Rasmussen, Torben Sølbeck, Streidl, Theresa, Hitch, Thomas C A, Wortmann, Esther, Deptula, Paulina, Kofoed, Michael V W, Riedel, Thomas, Neumann-Schaal, Meina, Hansen, Michael, Nielsen, Dennis Sandris, Clavel, Thomas, Vogensen, Finn Kvist
• Format: Journal Article
• Language: English
• Published: England 01.01.2021
• Subjects: acetate; succinate; spore-forming; Lachnospiraceae; mouse faeces; diet-induced obesity; Firmicutes
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.004673

A bacterial strain, designated WCA-9-b2 , was isolated from the caecal content of an 18-week-old obese C57BL/6NTac male mouse. According to phenotypic analyses, the isolate was rod-shaped, strictly anaerobic, spore-forming, non-motile and Gram-stain-positive, under the conditions tested. Colonies were irregular and non-pigmented. Analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the order with ATCC 27755 (94.9 % sequence identity), ATCC 29149 (94.8%) and ATCC 35704 (94.3%) being the closest relatives. Whole genome sequencing showed an average nucleotide identity <74.23 %, average amino acid identity <64.52-74.67 % and percentage of conserved proteins values <50 % against the nine closest relatives ( , , , , , , , and ). The genome-based G+C content of genomic DNA was 44.4 mol%. The major cellular fatty acids were C (24.5%), C 9 (19.8 %), C DMA (11.7%), C (8.4%) and C (6.6%). Respiratory quinones were not detected. The predominant metabolic end products of glucose fermentation were acetate and succinate. Production of CO and H were detected. Based on these data, we propose that strain WCA-9-b2 represents a novel species within a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain is WCA-9-b2 (=DSM 106039 =CECT 30156 ).