Jeotgalibaca dankookensis gen. nov., sp. nov., a member of the family Carnobacteriaceae, isolated from seujeot (Korean traditional food)

• Published in: International journal of systematic and evolutionary microbiology Vol. 64; no. Pt 5; pp. 1729 - 1735
• Main Authors: Lee, Dong-Geol, Trujillo, Martha E, Kang, Heecheol, Ahn, Tae-Young
• Format: Journal Article
• Language: English
• Published: England 01.05.2014
• Subjects: Bacterial Typing Techniques; Base Composition; Carnobacteriaceae - classification; Carnobacteriaceae - genetics; Carnobacteriaceae - isolation & purification; DNA, Bacterial - genetics; Fatty Acids - chemistry; Food Microbiology; Molecular Sequence Data; Peptidoglycan - chemistry; Phospholipids - chemistry; Phylogeny; Republic of Korea; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.057059-0

A novel, Gram-stain-positive bacterium, designated strain EX-07T, was isolated from seujeot (Korean traditional food). The strain was aerobic, halotolerant and non-motile; it formed cocci that grouped into tetrads and sarcinae or formed irregular conglomerates. Growth occurred at pH 7-9, at 10-37 °C and with up to 9% NaCl. Isolate EX-07T was catalase- and oxidase-negative and used sugars and organic acids as carbon sources. The 16S rRNA gene sequence of the novel strain showed 94.2-94.5% similarity with the type strains of Trichococcus pasteurii, Trichococcus patagoniensis, Trichococcus collinsii, Trichococcus flocculiformis and Trichococcus palustris and only 92.2% with representatives of the genera Bavariicoccus, Carnobacterium and Granulicatella. Sequence similarities based on the groEL gene ranged from 81.3 to 82.8% between the novel isolate and the type strains of all species of the genus Trichococcus, and only 74.2 and 75.3% with type strains of members of the genera Bavariicoccus and Granulicatella, respectively. The G+C content of the genomic DNA was 39.6 mol%. The predominant fatty acids were C16:1ω9c, C18:1ω9c, C16:0 and C14:0. The polar lipid profile was very complex and included phosphatidylethanolamine and several unidentified aminolipids, glycolipids and phospholipids. Based on the genotypic and phenotypic results obtained in this study, it is proposed that isolate EX-07T represents a novel species of a new genus in the family Carnobacteriaceae for which the name Jeotgalibaca dankookensis gen. nov., sp. nov. is proposed. The type strain of Jeotgalibaca dankookensis is EX-07T (=KCCM 90229T=JCM 19215T).