Emticicia aquatilis sp. nov., isolated from a freshwater sample

• Published in: International journal of systematic and evolutionary microbiology Vol. 67; no. 6; pp. 1703 - 1708
• Main Authors: Ngo, Hien T T, Trinh, Huan, Yang, Jung-Eun, Won, Kyung-Hwa, Chu, Dong-Hun, Kook, MooChang, Yi, Tae-Hoo
• Format: Journal Article
• Language: English
• Published: England 01.06.2017
• Subjects: Bacterial Typing Techniques; Base Composition; Cytophagaceae - classification; Cytophagaceae - genetics; Cytophagaceae - isolation & purification; DNA, Bacterial - genetics; Fatty Acids - chemistry; Fresh Water - microbiology; Glycolipids - chemistry; Nucleic Acid Hybridization; Phosphatidylethanolamines - chemistry; Phylogeny; Pigmentation; Republic of Korea; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; Republic of Korea
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.001840

A Gram-stain-negative, facultatively anaerobic, non-motile, rod-shaped and yellow-pigmented bacterium, designated strain THG-DN6.14T, was isolated from a freshwater sample near Donghaksa temple in Daejeon, South Korea. On the basis of the results of 16S rRNA gene sequence comparisons, THG-DN6.14T was found to be most closely related to Emticicia sediminis JBR12T (99.1 % sequence similarity), Emticicia oligotrophica DSM 17448T (97.6 %), Emticicia aquatica HMF2925T (96.5 %), and Emticicia ginsengisoliGsoil 085T (94.4 %). The DNA-DNA relatedness between THG-DN6.14T and its phylogenetically closest neighbours was below 65.0 %. The DNA G+C content was 43.3 mol%. The major polar lipids were found to be phosphatidylethanolamine, an unidentified glycolipid and an unidentified aminoglycolipid. The major fatty acids were identified as C16 : 0, iso-C15 : 0, iso-C17 : 0 3-OH, and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The respiratory quinone was menaquinone MK-7. These data supported the affiliation of THG-DN6.14T to the genus Emticicia. THG-DN6.14Tcould be distinguished from related species of the genus Emticicia by physiological and biochemical tests. Therefore, the novel isolate represents a novel species, for which the name Emticicia aquatilis sp. nov. is proposed, with THG-DN6.14T (=KACC 18540T=CGMCC 1.15958T) as the type strain.