Macroalgae as a source of sugar and detoxifier biochar for polyhydroxyalkanoates production by Halomonas sp. YLGW01 under the unsterile condition

• Published in: Bioresource technology Vol. 384; p. 129290
• Main Authors: Bhatia, Shashi Kant, Hwang, Jeong Hyeon, Oh, Suk Jin, Kim, Hyun Jin, Shin, Nara, Choi, Tae-Rim, Kim, Hyun-Joong, Jeon, Jong-Min, Yoon, Jeong-Jun, Yang, Yung-Hun
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.09.2023
• Subjects: Biochar; Bioplastic; Detoxification; Halomonas; Macroalgae; Polyhydroxyalkanoates; Unsterile; Halomonas; Unsterile; Detoxification; Macroalgae; Biochar; Polyhydroxyalkanoates; Bioplastic
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2023.129290

[Display omitted] •Macroalgal biomass is a sustainable and renewable feedstock for PHA production.•Halomonas sp. have the capability to utilize galactose and glucose and produce PHA.•Eucheuma spinosum derived biochar is used to detoxify its biomass hydrolysate.•Detoxified and unsterilized hydrolysate valorized into PHA by Halomonas sp. Macroalgae (seaweed) is considered a favorable feedstock for polyhydroxyalkanoates (PHAs) production owing to its high productivity, low land and freshwater requirement, and renewable nature. Among different microbes Halomonas sp. YLGW01 can utilize algal biomass-derived sugars (galactose and glucose) for growth and PHAs production. Biomass-derived byproducts furfural, hydroxymethylfurfural (HMF), and acetate affects Halomonas sp. YLGW01 growth and poly(3-hydroxybutyrate) (PHB) production i.e., furfural > HMF > acetate. Eucheuma spinosum biomass-derived biochar was able to remove 87.9 % of phenolic compounds from its hydrolysate without affecting sugar concentration. Halomonas sp. YLGW01 grows and accumulates a high amount of PHB at 4 % NaCl. The use of detoxified unsterilized media resulted in high biomass (6.32 ± 0.16 g cdm/L) and PHB production (3.88 ± 0.04 g/L) compared to undetoxified media (3.97 ± 0.24 g cdm/L, 2.58 ± 0.1 g/L). The finding suggests that Halomonas sp. YLGW01 has the potential to valorize macroalgal biomass into PHAs and open a new avenue for renewable bioplastic production.