Analysis of muscle cell culture medium by size-exclusion chromatography

The application of both low-pressure (preparative) and high-performance (analytical) size-exclusion chromatography to the fingerprinting of muscle cell culture supernatant is reported. The chromatograms showed significant differences between fresh media and muscle cell culture media. In addition, on...

Full description

Saved in:
Bibliographic Details
Published inJournal of chromatography. B, Biomedical applications Vol. 664; no. 1; pp. 185 - 191
Main Authors Dalla Libera, L., Massimino, M.L., Arslan, P., Beltrame, M., Cantini, M.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 03.02.1995
Subjects
Animals
Cell Division
Cercopithecus aethiops
Chromatography, Gel
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Culture Media - chemistry
Muscle Proteins - isolation & purification
Muscle Proteins - physiology
Muscles - cytology
Vero Cells
Online AccessGet full text

Cover

More Information
Summary:The application of both low-pressure (preparative) and high-performance (analytical) size-exclusion chromatography to the fingerprinting of muscle cell culture supernatant is reported. The chromatograms showed significant differences between fresh media and muscle cell culture media. In addition, only one fraction derived from muscle culture medium contained factor(s) of proteic nature able to interfere with the cell cycle of a continuous proliferating cell line.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0378-4347
1572-6495
DOI:10.1016/0378-4347(94)00349-A