Development of competitive enzyme-linked immunosorbent assays (ELISAs) based on monoclonal antibodies for chloronicotinoid insecticides imidacloprid and acetamiprid

• Published in: Analytica chimica acta Vol. 427; no. 2; pp. 211 - 219
• Main Authors: Wanatabe, Shigeyuki, Ito, Shigekazu, Kamata, Yoshio, Omoda, Naiki, Yamazaki, Tetsuo, Munakata, Hiroshi, Kaneko, Takashi, Yuasa, Yojiro
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 26.01.2001; Elsevier
• Subjects: Acetamiprid; Biological and medical sciences; DC-ELISA; Food industries; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; Imidacloprid; Immunoassay; Monoclonal antibody; Acetamiprid; Immunoassay; DC-ELISA; Monoclonal antibody; Imidacloprid; Insecticide; Analysis method; Fruit; Pesticides; Guanidines; ELISA assay; Vegetable; Quantitative analysis; Chemical contamination; Immunological method; Pyridine derivatives
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/S0003-2670(00)01126-0

Enzyme-linked immunosorbent assays (ELISAs) based on monoclonal antibodies (MoAbs) for chloronicotinoid insecticides imidacloprid and acetamiprid were developed. The MoAbs for either imidacloprid or acetamiprid were obtained utilizing mice with 3-[5-(2-nitroiminoimidazolidin-1-ylmethyl)-2-pyridylthio]propionic acid or 3-[5-( N-(1-cyanoiminoethyl)- N-methylaminomethyl)-2-pyridylthio] propionic acid haptens conjugated to bovine serum albumin, respectively. Both MoAbs (33C3-1-1 for imidacloprid and 45E10-1-1 for acetamiprid) characterized by an indirect competitive ELISA showed high sensitivity and low cross-reaction to related compounds. Direct competitive ELISAs (DC-ELISAs) with 33C3-1-1 for imidacloprid or with 45E10-1-1 for acetamiprid were tested on spiked vegetable and fruit samples without clean-up procedures. The mean recovery of the either assay measured by DC-ELISA ranged from 80 to 120% for imidacloprid or from 80 to 100% for acetamiprid, respectively. Both recovery tests for imidacloprid and acetamiprid showed good performance by DC-ELISA at the permitted level (0.5 ppm for imidacloprid and 1 ppm for acetamiprid) in vegetable and fruit. The result demonstrates that these assays could be easier to handle and as well as lesser expensive than usual high-performance assays such as gas chromatography (GC), high performance liquid chromatography (HPLC) and GC-mass spectrometry (GC-MS).