Callus and plantlet formation from cultured wheat anthers at different developmental stages

• Published in: Plant science letters Vol. 33; no. 1; pp. 71 - 79
• Main Authors: Ding-Gang, He, Jun-Wen, Ouyang
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 1984; Elsevier
• Subjects: Agronomy. Soil science and plant productions; Anther and ovule culture, utilization for haploid production; Anther culture; Anther stage; Biological and medical sciences; Biotechnology; Economic plant physiology; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; In vitro culture; Methods. Procedures. Technologies; Plant cells and fungal cells; Plant physiology and development; Pollen stage; Tissue cultures, protoplasts; Triticum aestivum; 2,4-D; Bi; PMC; MU; Tetr; Anther culture; LU; Tri; EU-I; EU-II; P; Pollen stage; Meio; Anther stage; Triticum aestivum; Anther; Monocotyledones; Callus; Gramineae; Angiospermae; Tissue culture; Plantlet; Spermatophyta; Pollen
• ISSN: 0304-4211
• DOI: 10.1016/0304-4211(84)90070-1

Wheat ( Triticum aestivum L.) anthers at different developmental stages were cultured on Potato-2 medium to test the ability of the pollen to produce calli. The frequency of anthers which produced calli was maximum when the anthers were excised at mid- or late uninucleate stage while the peak of yield of calli and green plantlets usually appeared at mid-uninucleate stage. The range of stages at which the anthers were productive varied with genotypes, however, calli and plantlets were obtained from anthers at pollen mother cell to trinucleate stage in two F 1 hybrids.