Effect of vitamin E succinate on the expression of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor in gastric cancer cells and CD4+ T cells

• Published in: Molecular bioSystems Vol. 11; no. 11; pp. 3119 - 3128
• Main Authors: Hou, Liying, Zhang, Haijin, Xu, Peixiang, Zhang, Lijia, Zhang, Xuguang, Sun, Yanpei, Huang, Xiaoli, Wu, Kun
• Format: Journal Article
• Language: English
• Published: England 01.01.2015
• Subjects: alpha-Tocopherol - pharmacology; Antineoplastic Agents - pharmacology; Apoptosis - drug effects; CD4-Positive T-Lymphocytes - drug effects; CD4-Positive T-Lymphocytes - metabolism; Cell Line, Tumor; Humans; Receptors, TNF-Related Apoptosis-Inducing Ligand - metabolism; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; TNF-Related Apoptosis-Inducing Ligand - metabolism
• ISSN: 1742-206X; 1742-2051
• DOI: 10.1039/c5mb00350d

Gastric malignancy, which shows poor prognosis, is one of the most frequent causes of cancer-associated deaths. Vitamin E succinate (VES) inhibits cell proliferation and induces apoptosis in a concentration- and time-dependent manner. We explored the effect of VES on the expression of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor in gastric cancer cells and CD4 + T cells. On one hand, VES dose-dependently regulated the expression of the TRAIL receptor in gastric cancer cells. Moreover, the activation of the TRAIL receptor, death receptor 4 (DR4), and death receptor 5 (DR5) in gastric cancer cells increased for up to 12 h. On the other hand, the expression of TRAIL protein in human CD4 + T cells was obviously upregulated in the presence of VES. On the basis of these findings, we combined VES and human CD4 + T cells to induce apoptosis of MKN28 human gastric cancer cells. The results showed that VES induced higher gastric cancer cell apoptosis when combined with human CD4 + T cells than when applied alone. We conclude that VES can induce the expression of TRAIL receptor in gastric cancer cells, as well as the expression of TRAIL in CD4 + T cells. Overall, our results provide a theoretical basis for future immunotherapy studies. Gastric malignancy, which shows poor prognosis, is one of the most frequent causes of cancer-associated deaths.