Proposal of Henriciella barbarensis sp. nov. and Henriciella algicola sp. nov., stalked species of the genus and emendation of the genus Henriciella

• Published in: International journal of systematic and evolutionary microbiology Vol. 67; no. 8; pp. 2804 - 2810
• Main Authors: Abraham, Wolf-Rainer, de Carvalho, Maira Peres, da Costa Neves, Thais Souto Paula, Memoria, Marina Torquato, Tartuci, Iago Toledo, Vancanneyt, Marc, Smit, John, Rohde, Manfred
• Format: Journal Article
• Language: English
• Published: England 01.08.2017
• Subjects: Alphaproteobacteria - classification; Alphaproteobacteria - genetics; Alphaproteobacteria - isolation & purification; Bacterial Typing Techniques; Base Composition; California; DNA, Bacterial - genetics; Fatty Acids - analysis; Glycolipids - chemistry; Nucleic Acid Hybridization; Phosphatidylglycerols - chemistry; Phylogeny; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Sequence Analysis, DNA; United States Virgin Islands
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.002024

Two Gram-negative, heterotrophic, aerobic, prosthecated, marine bacteria, designated strains MCS23T and MCS27T, were isolated from seawater samples. NaCl was required for growth. The major polar lipid detected in strain MCS27T was phosphatidylglycerol, whereas those detected in MCS23T were phosphatidylglycerol, sulfoquinovosyl diacylglycerol and 1,2-diacyl-3-α-d-glucuronopyranosyl-sn-glycerol taurineamide. The most abundant cellular fatty acids were C18 : 1ω7 and C16 : 0, hydroxyl-fatty acids were 3-OH C12 : 0 in both strains and 3-OH C11 : 0 in MCS23T. Strains MCS23T and MCS27T had DNA G+C contents of 57.0 and 55.0 mol%, respectively. The two strains shared 99.3 % 16S rRNA gene sequence similarity; levels of similarity with the type strains of species of the genus Henriciella were 99.4-97.8 % but DNA-DNA hybridizations were 53 % or lower. Besides their 16S rRNA gene sequences, the novel strains can be differentiated from other species of the genus Henriciella by cell morphology, lipid and fatty acid patterns and enzyme activities. The data obtained led to the identification of two novel species, for which the names Henriciella barbarensis sp. nov. (type strain MCS23T=LMG 28705T=CCUG 66934T) and Henriciella algicola sp. nov. (type strain MCS27T=LMG 29152T=CCUG 67844T) are proposed. As these two novel species are the first prosthecate species in the genus Henriciella, an emended genus description is also provided.