Agromyces protaetiae sp. nov., isolated from gut of larva of Protaetia brevitarsis seulensis

• Published in: International journal of systematic and evolutionary microbiology Vol. 70; no. 2; pp. 1259 - 1265
• Main Authors: Heo, Jun, Hamada, Moriyuki, Tamura, Tomohiko, Saito, Satomi, Lee, Soon Dong, Kim, Jeong-Seon, Hong, Seung-Beom, Kwon, Soon-Wo, Kim, Soo-Jin
• Format: Journal Article
• Language: English
• Published: England 01.02.2020
• Subjects: Actinobacteria - classification; Actinobacteria - isolation & purification; Animals; Bacterial Typing Techniques; Base Composition; Coleoptera - microbiology; DNA, Bacterial - genetics; Fatty Acids - chemistry; Gastrointestinal Tract - microbiology; Glycolipids - chemistry; Larva - microbiology; Peptidoglycan - chemistry; Phospholipids - chemistry; Phylogeny; Republic of Korea; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Vitamin K 2 - chemistry; Republic of Korea; Protaetia brevitarsis seulensis; Agromyces protaetiae; taxonomy; new taxa
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.003908

An actinobacterial strain, designated FW100M-8 , was isolated from a gut sample of larva of at the National Institute of Agricultural Sciences, Wanju-gun, South Korea. Cells were Gram-stain-positive, microaerophilic to aerobic, non-spore forming and non-motile. It grew at pH 7.0-9.0 (optimum, pH 8.0), at 15-35 °C (optimum, 28 °C) and 0-3.0 % (w/v) NaCl (optimum, 0 %). According to the 16S rRNA gene analysis, strain FW100M-8 shared the highest sequence similarity with DSM 20152 (98.4 %), XIL01 (98.3 %), NIO-1018 (98.3 %), MJ21 (98.3 %), and AK-1 (97.9 %). Phylogenetic trees showed that strain FW100M-8 fell into the lineage of the genus . The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid and an unidentified lipid. The menaquinones of strain FW100M-8 were MK-12 (46 %), MK-11 (36 %), MK-10 (14 %) and MK-13 (4 %). The major fatty acids were anteiso-C , anteiso-C and iso-C . The peptidoglycan type was supposed to be the type B1, comprising d-Ala, d-Glu, Gly and l-A bu. The G+C content of the genomic DNA is 70.5 mol%. On the basis of the genotypic and phenotypic data, we conclude that strain FW100M-8 represents a novel species of the genus , for which the name sp. nov. is proposed with strain FW100M-8 (=KACC 19308 =NBRC 113048 ) as the type strain.