Differentiating adult hippocampal stem cells into neural crest derivatives

• Published in: Neuroscience Vol. 118; no. 1; pp. 1 - 5
• Main Authors: ALEXANIAN, A. R, SIEBER-BLUM, M
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier 01.04.2003
• Subjects: Animals; Aves; Biological and medical sciences; Cell Communication - physiology; Cell Differentiation - physiology; Cells, Cultured - cytology; Cells, Cultured - metabolism; Chondrocytes - cytology; Chondrocytes - metabolism; Coculture Techniques - methods; Coculture Techniques - trends; Cues; Development. Senescence. Regeneration. Transplantation; Fundamental and applied biological sciences. Psychology; Hippocampus - cytology; Hippocampus - growth & development; Hippocampus - metabolism; Melanocytes - cytology; Melanocytes - metabolism; Mice; Myocytes, Smooth Muscle - cytology; Myocytes, Smooth Muscle - metabolism; Neural Crest - cytology; Neural Crest - metabolism; Neuronal Plasticity - physiology; Quail; Stem Cells - cytology; Stem Cells - metabolism; Vertebrates: nervous system and sense organs; ROSA26; smooth muscle cell; Stem cell; Central nervous system; Neural crest; In vitro; quail; chondrocyte; melanocyte; culture; Plasticity; Differentiation; Hippocampus; Brain (vertebrata)
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/S0306-4522(02)00994-6

To investigate the degree of plasticity of hippocampal neural stem cells from adult mice (mHNSC), we have analyzed their differentiation in co-culture with quail neural crest cells. In mixed culture, mHNSC give rise to several non-neuronal neural crest derivatives, including melanocytes, chondrocytes and smooth muscle cells. The data suggest that neural crest cell-derived short-range cues that are recognized across species can instruct adult mHNSC to differentiate into neural crest phenotypes.