Noviherbaspirillum aerium sp. nov., isolated from air

• Published in: International journal of systematic and evolutionary microbiology Vol. 70; no. 12; pp. 6390 - 6395
• Main Authors: Xue, Han, Piao, Chun-Gen, Lin, Ying-Hua, Bian, Dan-Ran, Li, Yong
• Format: Journal Article
• Language: English
• Published: England 01.12.2020
• Subjects: Air Microbiology; Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial - genetics; Fatty Acids - chemistry; Oxalobacteraceae - classification; Oxalobacteraceae - isolation & purification; Phospholipids - chemistry; Phylogeny; Pigmentation; Putrescine - chemistry; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Ubiquinone - chemistry; China; strain 122213-3T; Noviherbaspirillum aerium sp. nov; taxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.004537

A Gram-stain-negative, strictly aerobic, non-spore-forming, rod-shaped, motile with polar flagella and pale-orange bacterium, designated strain 122213-3 , was isolated from air, collected at the foot of the Xiangshan Mountain, located in Beijing, PR China. Optimal growth occurred at 28 °C, at pH 7 and in the presence of 0-1 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that 122213-3 clustered with species of the genus and formed a distinct sublineage, showing highest similarities to CC-AFH3 (96.88 %), JC206 (95.78 %) and SUEMI08 (95.78 %). The predominant cellular fatty acids were summed feature 3 (C 6 and/or C 7 ), summed feature 8 (C 7 and/or C 6 ) and C . The predominant quinone was ubiquinone 8 (Q-8). The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, unidentified phospholipid and two unidentified polar lipids. The polyamine pattern showed the presence of putrescine as the major polyamine, with minor amounts of 2-hydroxyputrescine. The DNA G+C content was 60.1 mol%. The phylogenetic analysis and physiological and biochemical data showed that strain 122213-3 should be classified as representing a novel species in the genus , for which the name sp. nov. is proposed. The type strain of is 122213-3 (=CFCC 14286 =LMG 30131 ).