Deinococcus ruber sp. nov., a radiation-resistant bacterium isolated from soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 67; no. 1; pp. 72 - 76
• Main Authors: Kim, Eun Bit, Kang, Myung Suk, Joo, Eun Sun, Jeon, Seon Hwa, Jeong, Sun Wook, Lim, Sang Yong, Jung, Hee Young, Srinivasan, Sathiyaraj, Kim, Myung Kyum
• Format: Journal Article
• Language: English
• Published: England 01.01.2017
• Subjects: Bacterial Typing Techniques; Base Composition; Deinococcus - classification; Deinococcus - isolation & purification; Deinococcus - radiation effects; DNA, Bacterial - genetics; Fatty Acids - chemistry; Gamma Rays; Gardens; Glycolipids - chemistry; Phospholipids - chemistry; Phylogeny; Pigmentation; Republic of Korea; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Ultraviolet Rays
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.001567

Two gamma- and UVC-resistant bacterial strains, designated JSH3-1T and 9-2-2, were isolated from garden soil in South Korea. Cells were Gram-stain-positive, aerobic, non-motile and spherical. A polyphasic approach was used to study the taxonomic properties of strains JSH3-1T and 9-2-2. Phylogenetic analysis based on nearly full-length 16S rRNA gene sequences of strains JSH3-1T and 9-2-2 indicated highest similarity with Deinococcus radiomollis PO-04-20-132T (94.7 and 94.9 %, respectively); levels of sequence similarity with the type strains of other Deinococcus species were less than 94.0 %. Strains JSH3-1T and 9-2-2 shared relatively high 16S rRNA gene sequence similarity (98.7 %) and had a high DNA reassociation value of 81±0.5 %. Meanwhile, they showed low levels of DNA reassociation (<25 %) with other closely related species of the genus Deinococcus. The two strains showed chemotaxonomic features typical of the genus Deinococcus, with the presence of menaquinone 8 as the respiratory quinone. The predominant fatty acids were iso-C17 : 0, iso-C13 : 0 and anteiso-C13 : 0. The polar lipids comprised phosphoglycolipid, aminophospholipid, glycolipid and unknown aminolipids. The DNA G+C contents of strains JSH3-1T and 9-2-2 were 62.0 and 61.9 mol%, respectively. On the basis of their phenotypic and genotypic characteristics, and phylogenetic distinction, strains JSH3-1T (=KCTC 33790T=JCM 31311T) and 9-2-2 (=KCTC 33789=JCM 31310) should be classified within a novel species of the genus Deinococcus, for which the name Deinococcus ruber sp. nov. is proposed.