Bacteroides caecicola sp. nov. and Bacteroides gallinaceum sp. nov., isolated from the caecum of an Indonesian chicken

• Published in: International journal of systematic and evolutionary microbiology Vol. 66; no. 3; pp. 1431 - 1437
• Main Authors: Irisawa, Tomohiro, Saputra, Sugiyono, Kitahara, Maki, Sakamoto, Mitsuo, Sulistiani, Yulineri, Titin, Dinoto, Achmad, Ohkuma, Moriya
• Format: Journal Article
• Language: English
• Published: England 01.03.2016
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.000899

Six strains of anaerobic bacteria, C13EG70 , C13EG118, C13EG186 , C13GAMG5, C13GAMG28 and C13GAMG40, were isolated from the caecum of a healthy chicken bred in Bogor, Indonesia. Phylogenetic analysis showed the isolates were separated into two groups. Group I (C13EG70 and C13EG118) showed nearly identical 16S rRNA gene sequences (99.9 % sequence similarity). Group II (C13EG186 , C13GAMG5, C13GAMG28 and C13GAMG40) showed nearly identical 16S rRNA gene sequences (>99.4 % sequence similarity). The isolates showed low 16S rRNA gene sequence similarities to recognized species of the genus . High gene sequence similarities were found between type strains (C13EG70 and C13EG186 ) and JCM 13657 (87.9, 91.5 %, respectively). Physiological, biochemical and genotypic characteristics demonstrated that these strains could be separated from the type strain of . It is concluded that Group I and Group II represent novel species. Two novel species of the genus are proposed as sp. nov. (type strain C13EG70  = LIPI12-4-Ck732  = JSAT12-4-Ck732  = InaCC B449  = NBRC 110958 ) and sp. nov. (type strain C13EG186  = LIPI12-4-Ck844  = JSAT12-4-Ck884  = InaCC B451  = NBRC 110963 ).