Stereological Method in Optical Coherence Tomography for In Vivo Evaluation of Laser-Induced Choroidal Neovascularization

• Published in: Ophthalmic surgery, lasers & imaging Vol. 49; no. 9; pp. e65 - 74
• Main Authors: Trujillo-Sanchez, Gloria Paulina, Martinez-Camarillo, Juan Carlos, Spee, Christine K, Hinton, David R, Humayun, Mark S, Weitz, Andrew C
• Format: Journal Article
• Language: English
• Published: United States SLACK INCORPORATED 01.09.2018
• Subjects: Lasers; Macular degeneration; Medical imaging; Retina; Tomography; Los Angeles California
• ISSN: 2325-8160; 2325-8179
• DOI: 10.3928/23258160-20180907-09

To evaluate a stereological method in optical coherence tomography (OCT) as an in vivo volume measurement of laser-induced choroidal neovascularization (L-CNV) lesion size. Laser photocoagulation was applied in rats to rupture Bruch's membrane and induce L-CNV. In vivo OCT images of neovascular lesions were acquired with a spectral-domain OCT system at days 0, 3, 7, 10, and 14 after laser surgery. A stereological image-processing method was used to calculate lesion volumes from the OCT images. Rats were euthanized at day 14, and confocal microscopy was used to obtain accurate volume measurements of the lesions ex vivo. Lesion sizes calculated from OCT and confocal were compared. In vivo assessment by OCT allowed three distinct stages of L-CNV to be visualized: the initial early reaction, neovascular proliferation, and regression. At day 14, correlations between OCT and confocal lesion volumes showed a positive association (Pearson's r = 0.50, P < .01). Except for the largest lesions, volumes measured by OCT were statistically similar to those measured by the confocal gold standard (P = .90). The stereological approach used to measure neovascular lesion volume from OCT images offers an accurate means to track L-CNV lesion size in vivo. [Ophthalmic Surg Lasers Imaging Retina. 2018;49:e65-e74.].