Potential limitations of the Sleeping Beauty transposon use in gene expression studies

MCPIP2 is the least known member of the MCPIP family of proteins. Recently we have found that it is a new RNase involved in transcript turnover. However, the full spectrum of its cellular targets is still unidentified. To discover transcripts which are regulated by this protein we have employed Slee...

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Bibliographic Details
Published inActa biochimica Polonica Vol. 66; no. 3; p. 263
Main Authors Sowińska, Weronika, Wawro, Mateusz, Solecka, Aleksandra, Kasza, Aneta
Format Journal Article
LanguageEnglish
Published Poland 12.07.2019
Subjects
Aged
Brain Neoplasms - genetics
Brain Neoplasms - pathology
Cell Line, Tumor
CRISPR-Cas Systems - genetics
DNA Transposable Elements - genetics
Gene Editing
Gene Knockout Techniques
Glioblastoma - genetics
Glioblastoma - pathology
Humans
Interleukin-6 - genetics
Male
Mutagenesis, Insertional - methods
Ribonucleases - genetics
RNA, Messenger - genetics
Transcription Factors - genetics
Transcriptome - genetics
Transfection
Vascular Endothelial Growth Factor A - genetics
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Summary:MCPIP2 is the least known member of the MCPIP family of proteins. Recently we have found that it is a new RNase involved in transcript turnover. However, the full spectrum of its cellular targets is still unidentified. To discover transcripts which are regulated by this protein we have employed Sleeping Beauty transposons. This tool allows for rapid generation of a stable transgenic cell line with inducible expression of the desired gene. In this study, we analysed how the Sleeping Beauty system itself influences expression of chosen genes, namely IL-6, Regnase-1 and VEGF. We found that the system alone may influence expression of IL-6. Our results indicate that Sleeping Beauty transposons should be used with caution in studies that are focused on changes in the transcript level.
ISSN:0001-527X
1734-154X
DOI:10.18388/abp.2019_2839