Kordia zhangzhouensis sp. nov., isolated from surface freshwater

• Published in: International journal of systematic and evolutionary microbiology Vol. 65; no. 10; pp. 3379 - 3383
• Main Authors: Du, Juan, Liu, Yang, Lai, Qiliang, Dong, Chunming, Xie, Yanrong, Shao, Zongze
• Format: Journal Article
• Language: English
• Published: England 01.10.2015
• Subjects: Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial - genetics; Fatty Acids - chemistry; Flavobacteriaceae - classification; Flavobacteriaceae - genetics; Flavobacteriaceae - isolation & purification; Glycolipids - chemistry; Molecular Sequence Data; Nucleic Acid Hybridization; Phospholipids - chemistry; Phylogeny; Pigmentation; Rivers - microbiology; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; Water Microbiology
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.000424

An aerobic, Gram-stain-negative, rod-shaped and non-motile bacterium, JS14SB-1T, was isolated from the surface freshwater of the Jiulong River, PR China. Strain JS14SB-1T grew at 15–38 °C (optimum, 28–35 °C), at pH 6.0–9.0 (optimum pH 7.0) and in the presence of 1.0–7.0 % (w/v) NaCl [optimum 3.0–5.0 % (w/v)]. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain JS14SB-1T was affiliated to the genus Kordia, sharing low similarities (95.1–97.1 %) to all type strains of species of this genus. The digital DNA–DNA hybridization (DDH) value between strain JS14SB-1T and the closely related strain Kordia jejudonensis SSK3-3T was 20.70 ± 2.33 % and far below the 70 % DDH value taken as the gold standard for delineation of bacterial species. The major fatty acids were identified as iso-C15 : 0 and iso-C17 : 0 3-OH. The polar lipids were phosphatidylethanolamine, glycolipid, aminolipid, several unidentified phospholipids and lipids. The predominant menaquinone was MK-6. The G+C content of the genomic DNA was 33.8 mol%. Based on the phenotypic, phylogenetic and chemotaxonomic distinctiveness, strain JS14SB-1T is considered to represent a novel species of the genus Kordia, for which the name Kordia zhangzhouensis sp. nov. is proposed; the type strain is JS14SB-1T ( = MCCC 1A00726T = KCTC 42140T).