NIR-II fluorescence lateral flow immunosensor based on efficient energy transfer probe for point-of-care testing of tumor biomarkers

• Published in: Chinese chemical letters Vol. 36; no. 1; pp. 109834 - 473
• Main Authors: Song, Zhaorui, Hao, Qiulian, Li, Bing, Yuan, Yuwei, Zhang, Shanshan, Suo, Yongkuan, Han, Hai-Hao, Cheng, Zhen
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.01.2025; State Key Laboratory of Drug Research,Molecular Imaging Center,Shanghai Institute of Materia Medica,Shanghai 201203,China; University of Chinese Academy of Sciences,Beijing 100049,China%Shandong Laboratory of Yantai Drug Discovery,Bohai Rim Advanced Research Institute for Drug Discovery,Yantai 264117,China; Pharmacy College,Shandong First Medical University & Shandong Academy of Medical Sciences,Ji'nan 250117,China; Shandong Laboratory of Yantai Drug Discovery,Bohai Rim Advanced Research Institute for Drug Discovery,Yantai 264117,China%Institute of Molecular Medicine Joint Laboratory for Molecular Medicine Northeastern University,Shenyang 110000,China%Pharmacy College,Shandong First Medical University & Shandong Academy of Medical Sciences,Ji'nan 250117,China%Shandong Laboratory of Yantai Drug Discovery,Bohai Rim Advanced Research Institute for Drug Discovery,Yantai 264117,China; University of Chinese Academy of Sciences,Beijing 100049,China; Institute of Molecular Medicine Joint Laboratory for Molecular Medicine Northeastern University,Shenyang 110000,China
• Subjects: Donor-acceptor energy transfer (ET); Lateral flow immunoassay (LFA); Poly(lactic acid-co-glycolic acid) (PLGA); Rare-earth nanoparticles (RENPs); The second near-infrared (NIR-II) fluorescence; Tumor biomarker; Donor-acceptor energy transfer (ET); Poly(lactic acid-co-glycolic acid) (PLGA); Tumor biomarker; Lateral flow immunoassay (LFA); The second near-infrared (NIR-II) fluorescence; Rare-earth nanoparticles (RENPs); The second near-infrared(NIR-Ⅱ); fluorescence; Poly(lactic acid-co-glycolic acid)(PLGA); Donor-acceptor energy transfer(ET); Lateral flow immunoassay(LFA); Rare-earth nanoparticles(RENPs)
• ISSN: 1001-8417
• DOI: 10.1016/j.cclet.2024.109834

Fluorescence lateral flow immunoassay (LFA) has emerged as a powerful tool for rapid screening of various biomarkers owing to its simplicity, sensitivity and flexibility. It is noteworthy that fluorescent probe mainly determines the analytical performance of LFA. Due to the emission and excitation wavelengths are located in the visible region, most fluorophores are inevitably subject to light scattering and background autofluorescence. Herein, we reported a novel LFA sensor based on the second near-infrared (NIR-II) fluorescent probe with excellent anti-interference capability. The designed NIR-II probe was the Nd3+ and Yb3+ doped rare earth nanoparticles (RENPs) by employing Nd3+ as energy donor and Yb3+ as energy acceptor, which of the donor-acceptor energy transfer (ET) efficiency reached up to 80.7%. Meanwhile, relying on the convenient and effective encapsulation strategy of poly(lactic-co-glycolic acid) (PLGA) microspheres to RENPs, the surface functionalized NIR-II probe (RE@PLGA) was obtained for subsequent bioconjugation. Benefiting from the optical advantages of NIR-II probe, this proposed NIR-II LFA displayed a good linear relationship ranging from 7 ng/mL to 200 ng/mL for the detection of α-fetoprotein (AFP), an important biomarker of hepatocellular carcinoma (HCC). The limit of detection (LOD) was determined as low as 3.0 ng/mL, which was of 8.3 times lower than clinical cutoff value. It is promising that LFA sensor based on this efficient RENPs probe provides new opportunities for high sensitive detection of various biomarkers in biological samples. A NIR-II fluorescent probe (RENPs) with high energy transfer (ET) efficiency was designed by employing Nd3+ as energy donor and Yb3+ as energy acceptor. Then, a novel LFA sensor based on the efficient RENPs probe was constructed to sensitively detect tumor biomarkers. [Display omitted]