Histone expressions in mouse-rat somatic reconstituted cells

• Published in: Experimental cell research Vol. 161; no. 1; pp. 141 - 149
• Main Authors: Ajiro, Kozo, Nishikawa, Yasuhiro, Tosu, Mariko, Sekiguchi, Toyozo
• Format: Journal Article
• Language: English
• Published: Orlando, FL Elsevier Inc 01.11.1985; Elsevier
• Subjects: Animals; Biological and medical sciences; Cell Division - drug effects; Cell Line; Cell metabolism, cell oxidation; Cell Nucleus - physiology; Cell physiology; Chromatin - physiology; Cytoplasm - physiology; Fundamental and applied biological sciences. Psychology; Histones - analysis; Histones - biosynthesis; Hybrid Cells - metabolism; Mice; Molecular and cellular biology; Phenotype; Rats; Species Specificity; Tetradecanoylphorbol Acetate - pharmacology; Cell culture; Vertebrata; Mammalia; Hybrid cell; Rat; Mouse; Rodentia; Gene expression; Histone; Metabolism
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1016/0014-4827(85)90498-7

A critical analysis of histone expression was performed on the four interspecific and the two intraspecific reconstituted cells formed between karyoplast from mouse B 16 cells and the cytoplast from rat cells (L 6TG.CAP r) or mouse cells (B 82.CAP r). All the reconstituted cells had the same pattern of mouse histones and the same amount of mouse-specific H2B. 2 histone as that of mouse nuclear donor cells. A hybrid between B 16 and L 6TG.CAP r contained both mouse and rat-specific H1b sybtypes, whereas no rat-specific H1b was detected in the interspecific reconstituted cells. In both intra- and interspecific reconstituted cells, the proportion of H1b content was lower than that of B 6 cells but that of H1∘ was higher, indicating that the mouse H1 patterns from these cells slightly resembled the pattern of slower growing and differentiated cytoplast donor cells. As an effect of the tumor promoter, the H1 pattern tended to revert to that of the nuclear donor cells in agreement with the phenotypic reversion, without any significant change in cell growth.