Paraburkholderia dinghuensis sp. nov., isolated from soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 69; no. 6; pp. 1613 - 1620
• Main Authors: Fu, Jia-Cheng, Lv, Ying-Ying, You, Jia, Gao, Zeng-Hong, Wang, Bo-Feng, Qiu, Li-Hong
• Format: Journal Article
• Language: English
• Published: England 01.06.2019
• Subjects: Paraburkholderia; dinghuensis; taxonomy; proteobateria
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.003367

A novel Gram-stain-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterial strain, designated DHOA04 , was isolated from a forest soil sample collected at Dinghushan Biosphere Reserve, Guangdong Province, PR China (112° 31' E 23° 10' N). It grew optimally at 28-33 °C and pH 6.5-7.0. Strain DHOA04 contained Q-8 as the major respiratory quinone. Its main fatty acids were C16 : 0, C17 : 0cyclo, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). The DNA G+C content of DHOA04 was 63.0 mol%, which is in the range of the genus Paraburkholderia. The average nucleotide identity and digital DNA-DNA hybridization values for the complete genomes were 81.6-83.0 and 25.5-27.0 % between strain DHOA04 and five closely related type strains. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and two unidentified aminophospholipids. On the basis of 16S rRNA gene sequence analysis, the strain was found to be closely related to members of the genus Paraburkholderia, but clearly separated from the established species. Phylogenetic analysis based on the 16S rRNA gene sequences using the maximum-likelihood algorithm indicated that strain DHOA04 was most closely related to Paraburkholderia ferrariae NBRC 106233 . The phenotypic, chemotaxonomic and phylogenetic data, and genome analysis showed that strain DHOA04 represents a novel species of the genus Paraburkholderia, for which the name Paraburkholderia dinghuensis sp. nov. is proposed. The type strain is DHOA04 (=KCTC 42627 =LMG 28839 ).