The measurement of drug-induced calcium changes in vascular smooth muscle using X-ray microanalysis of pyroantimonate precipitates

A method is described for the X-ray microanalysis of calcium pyroantimonate precipitates in smooth muscle using an electron microscope. Stimulation of calcium influx through voltage-operated channels using KCl and blockade of this effect with nifedipine were used to validate the method. Significant...

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Bibliographic Details
Published inJournal of pharmacological methods Vol. 18; no. 1; p. 55
Main Authors Baker, J R, Haigh, R J, Warburton, G, Weston, A H, Williamson, I H
Format Journal Article
LanguageEnglish
Published United States 01.08.1987
Subjects
Animals
Antimony - analysis
Calcium - analysis
Chemical Precipitation
Electron Probe Microanalysis
Glutaral - pharmacology
Hydralazine - pharmacology
Male
Muscle, Smooth, Vascular - analysis
Nifedipine - pharmacology
Rats
Rats, Inbred Strains
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Summary:A method is described for the X-ray microanalysis of calcium pyroantimonate precipitates in smooth muscle using an electron microscope. Stimulation of calcium influx through voltage-operated channels using KCl and blockade of this effect with nifedipine were used to validate the method. Significant changes in the calcium content of rat renal artery were detected when osmium tetroxide-pyroantimonate were used as fixatives, whereas addition of glutaraldehyde yielded unsatisfactory data. Some effects of hydralazine are also briefly described.
ISSN:0160-5402
DOI:10.1016/0160-5402(87)90017-9