Ability of Lactobacillus plantarum LS/07 to modify intestinal enzymes activity in chronic diseases prevention

• Published in: Acta biochimica Polonica Vol. 64; no. 1; pp. 113 - 116
• Main Authors: Hijová, Emília, Kuzma, Jozef, Strojný, Ladislav, Bomba, Alojz, Bertková, Izabela, Chmelárová, Anna, Hertelyová, Zdena, Benetinová, Veronika, Štofilová, Jana, Ambro, Ľuboš
• Format: Journal Article
• Language: English
• Published: Poland 2017
• Subjects: Animals; Anti-Bacterial Agents - therapeutic use; Atherosclerosis - therapy; Bacteria - enzymology; Bacterial Proteins - metabolism; beta-Galactosidase - metabolism; beta-Glucosidase - metabolism; Combined Modality Therapy - methods; Dysbiosis - therapy; Glucuronidase - metabolism; Intestines - microbiology; Lactobacillus plantarum - physiology; Male; Neoplasms - therapy; Probiotics - therapeutic use; Rats; Rats, Sprague-Dawley; atherosclerosis; bacterial enzyme; Sprague-Dawley rats; cancer; dysbiosis
• ISSN: 0001-527X; 1734-154X
• DOI: 10.18388/abp.2016_1308

The ability of probiotic strain Lactobacillus plantarum LS/07 to modify the activity of intestinal bacterial enzymes - β-glucuronidase (β-GLUCUR), β-galactosidase (β-GAL), and β-glucosidase (β-GLU) in prevention of chronic diseases - cancer, atherosclerosis and dysbiosis was investigated. The male Sprague-Dawley rats were randomly divided into 12 experimental groups: controls groups - C (control), AT (atherosclerotic), CC (carcinogenic), dysbiotic groups - each group in combination with antibiotics (ATB), probiotics groups - in combinatioan with probiotic (PRO) alone, and each group with combination of antibiotic and probiotic (ATB+PRO). In the control group the β-glucuronidase activity did not change throughout the experiment. High fat diet in atherosclerotic group significantly increased the activity of β-glucuronidase (P<0.001) and β-glucosidase (P<0.01). Azoxymethane application in carcinogenic group significantly increased β-glucuronidase (P<0.01), but reduced β-glucosidase (P<0.01) activity. Daily application of probiotics alone and in combination with antibiotic increased β-galactosidase, of β-glucosidase, and decreased β-glucuronidase activity. In control antibiotic group we observed significant increase in β-glucuronidase (P<0.05) and decreased β-glucosidase (P<0.01) activity which can be caused by the change of microflora in favor of coliform bacteria. These findings indicate the positive effects of probiotic Lactobacillus plantarum LS/07 and suggest its use in disease prevention in human medicine and some animal species.