Sphingomonas rhizophila sp. nov., isolated from rhizosphere of Hibiscus syriacus

• Published in: International journal of systematic and evolutionary microbiology Vol. 68; no. 2; pp. 681 - 686
• Main Authors: Yan, Zheng-Fei, Lin, Pei, Won, Kyung-Hwa, Li, Chang-Tian, Park, GyungSoo, Chin, ByungSun, Kook, MooChang, Wang, Qi-Jun, Yi, Tae-Hoo
• Format: Journal Article
• Language: English
• Published: England 01.02.2018
• Subjects: Bacterial Typing Techniques; Base Composition; DNA, Bacterial - genetics; Fatty Acids - chemistry; Glycolipids - chemistry; Hibiscus - microbiology; Phospholipids - chemistry; Phylogeny; Republic of Korea; Rhizosphere; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Spermidine - chemistry; Sphingomonas - classification; Sphingomonas - genetics; Sphingomonas - isolation & purification; Ubiquinone - chemistry; Hibiscus syriacus; Sphingomonas rhizophila; rhizosphere
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.002566

A Gram-stain-negative, aerobic, non-motile, rod-shaped, catalase-positive and oxidase-positive bacteria (THG-T61 ), was isolated from rhizosphere of Hibiscus syriacus. Growth occurred at 10-37 °C (optimum 25-30 °C), at pH 5.0-9.0 (optimum 7.0) and in the presence of 0-2.0 % NaCl (optimum without NaCl supplement). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T61 were identified as Sphingomonas ginsengisoli KCTC 12630 (97.9 %), Sphingomonas jaspsi DSM 18422 (97.8 %), Sphingomonas astaxanthinifaciens NBRC 102146 (97.4 %), Sphingomonassediminicola KCTC 12629 (97.2 %), 'Sphingomonas swuensis' KCTC 12336 (97.1 %) and Sphingomonas daechungensis KCTC 23718 (96.9 %). The isoprenoid quinone was ubiquinone-10 (Q-10). The major fatty acids were C16 : 0, C17 : 1ω6c, summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1ω7c) and summed feature 7 (C18 : 1ω7c, C18 : 1ω9t and/or C18 : 1ω12t). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, one unidentified lipid, one unidentified phospholipid, one unidentified glycolipid and one unidentified phosphoglycolipid. The polyamine was homospermidine. The DNA G+C content of strain THG-T61 was 65.6 mol%. The DNA-DNA relatedness values between strain THG-T61 and its closest reference strains were less than 49.2 %, which is lower than the threshold value of 70 %. Therefore, strain THG-T61 represents a novel species of the genus Sphingomonas, for which the name Sphingomonas rhizophila sp. nov. is proposed. The type strain is THG-T61 (=KACC 19189 =CCTCC AB 2016245 ).