Group II Introns: Highly Structured yet Dynamic

RNA splicing, the removal of introns and ligation of exons, is a crucial process during mRNA maturation. Group II introns are large ribozymes that self-catalyze their splicing, as well as their transposition. They are living fossils of spliceosomal introns and eukaryotic retroelements. The yeast mit...

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Bibliographic Details
Published inChimia Vol. 77; no. 4; pp. 235 - 241
Main Authors Ahunbay, Esra, Zelger-Paulus, Susann, Sigel, Roland K. O.
Format Journal Article
LanguageEnglish
Published Swiss Chemical Society 26.04.2023
Subjects
Group II intron
RNA catalysis
RNA folding
RNA labeling
RNA splicing
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Summary:RNA splicing, the removal of introns and ligation of exons, is a crucial process during mRNA maturation. Group II introns are large ribozymes that self-catalyze their splicing, as well as their transposition. They are living fossils of spliceosomal introns and eukaryotic retroelements. The yeast mitochondrial Sc.ai5γ is the first identified and best-studied self-splicing group II intron. A combination of biochemical, biophysical, and computational tools enables studying its catalytic properties, structure, and dynamics, while also serving to develop new therapeutic and biotechnological tools. We survey the history of group II intron studies paralleling the trends in RNA methodology with Sc.ai5γ in the spotlight.
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ISSN:0009-4293
2673-2424
DOI:10.2533/chimia.2023.235