Multiple shoot regeneration from immature embryo explants of papaya

A simple and rapid method for multiple shoot formation in vitro from immature embryo axis explants of Carica papaya cvs. Honey Dew, Washington and Co2 is described. Multiple shoot regeneration was achieved by culture of the explants on modified Murashige and Skoog (MS) medium supplemented either wit...

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Published inBiologia plantarum Vol. 47; no. 3; pp. 327 - 331
Main Authors Bhattacharya, J, Renukdas, N.N, Khuspe, S.S, Rawal, S.K. (National Chemical Lab., Pune (India). Plant Tissue Culture Div.)
Format Journal Article
LanguageEnglish
Published 01.11.2003
Subjects
6-BENZILADENINA
6-BENZYLADENINE
AIB
ANA
BROTES
CARICA PAPAYA
EMBRIONES VEGETALES
EMBRYON VÉGÉTAL
IBA
IN VITRO REGENERATION
NAA
PLANT EMBRYOS
POUSSE
REGENERACIÓN IN VITRO
RÉGÉNÉRATION IN VITRO
SHOOTS
VITROPLANT
VITROPLANTAS
VITROPLANTS
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Summary:A simple and rapid method for multiple shoot formation in vitro from immature embryo axis explants of Carica papaya cvs. Honey Dew, Washington and Co2 is described. Multiple shoot regeneration was achieved by culture of the explants on modified Murashige and Skoog (MS) medium supplemented either with thidiazuron (TDZ; 0.45-22.7 microM) or a combination of benzylaminopurine (BAP; 0.2-8.84 microM) and naphthalene acetic acid (NAA; 0.5-2.64 microM). Highest frequency of shoot regeneration occurred on medium supplemented either with 2.25 microM TDZ or a combination of BAP (4.4 microM) and NAA (0.5 microM). Composition of the basal media influenced the frequency of multiple shoot initiation. Stunted shoots regenerated at 4.5 microM and higher concentrations of TDZ. Such shoots could, however, be elongated by transfer to medium containing 5.7 microM GA3. Rooting of the regenerated shoots was achieved in presence of indolebutyric acid (IBA; 4.92-19.68 microM), however, least response was in presence of 14.7 microM IBA. Rooted plants were hardened and transferred to pots.
Bibliography:F02
2004000241
ISSN:0006-3134
1573-8264
DOI:10.1023/B:BIOP.0000023873.55327.fe