In vitro precipitation of calcium oxalate in the presence of whole matrix or lipid components of the urinary stones

Organic matrix of human calcium oxalate urinary stones was obtained by demineralizing with EDTA. Lipids were extracted from the EDTA-insoluble matrix by chloroform methanol treatment. The whole matrix and its total lipid extract were then incubated in a metastable solution of calcium oxalate and dep...

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Bibliographic Details
Published inThe Journal of urology Vol. 139; no. 2; p. 418
Main Authors Khan, S R, Shevock, P N, Hackett, R L
Format Journal Article
LanguageEnglish
Published United States 01.02.1988
Subjects
Calcium Oxalate - analysis
Chemical Phenomena
Chemical Precipitation
Chemistry
Crystallization
Humans
Lipids - analysis
Microscopy, Electron, Scanning
Urinary Calculi - analysis
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Summary:Organic matrix of human calcium oxalate urinary stones was obtained by demineralizing with EDTA. Lipids were extracted from the EDTA-insoluble matrix by chloroform methanol treatment. The whole matrix and its total lipid extract were then incubated in a metastable solution of calcium oxalate and depletion of calcium and oxalate ions from the calcifying solution was determined. Results of our studies described here show that urinary calcium oxalate stone matrix and its total lipid contents were capable of binding calcium and oxalate ions and of catalysing calcium oxalate crystal formation from a metastable calcium oxalate solution.
ISSN:0022-5347
DOI:10.1016/S0022-5347(17)42447-5