The L1 Retroelement-related p40 Protein Induces p38δ MAP Kinase

• Published in: Autoimmunity (Chur, Switzerland) Vol. 37; no. 1; pp. 57 - 65
• Main Authors: Kuchen, Stefan, Seemayer, Christian A., Rethage, Janine, Knoch, Rebecca von, Kuenzler, Peter, Michel, Beat A., Gay, Renate E., Gay, Steffen, Neidhart, Michel
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 01.02.2004; Taylor and Francis
• Subjects: 1-retrotransposon; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Fundamental immunology; General aspects; Immunopathology; Medical sciences; p38δ; Rheumatoid arthritis; Synovial fibroblasts; Autoimmunity; Synovial fibroblasts; Cartography; Immunopathology; Enzyme; Transferases; Diseases of the osteoarticular system; Mitogen-activated protein kinase; Autoimmune disease; Inflammatory joint disease; EC 2.7.1.37; p38δ; Chronic; Maps; Immunology; Synovial membrane; Protein kinase; Rheumatoid arthritis; 1retrotransposon; Microtubule associated protein; Fibroblast
• ISSN: 0891-6934; 1607-842X
• DOI: 10.1080/08916930310001637977

We characterized a full length L1 mRNA in a rheumatoid arthritis (RA) synovial tissue and determined the degree of methylation of its 5′-UTR. We asked whether not only intact but also altered L1s can exert biological activities by transfecting RA synovial fibroblasts (SF) with either retrotransposition-competent or incompetent L1s and examined their capacity to induce p38δ. Total RNA was isolated from the synovial tissue of a 35-year-old woman with highly destructive RA. A complete L1 sequence was obtained by 3′/5′-RACE. Methylation of the genomic 5′-UTR was determined by the sodium-disulfide/PCR method. RA-SF were transfected by lipofection with either a functional L1 or an ORF2-mutated L1 element. The expression of p38δ was measured by RT-PCR and Western blot. The full length L1 mRNA included a 5′-UTR, an ORF1 and an ORF2. Three of five CpG islands (60%) of the genomic L1 5′-UTR were hypomethylated and the ORF2 was deactivated by the insertion of stop codons. Both, intact and ORF2-mutated L1 vectors, induced the expression of p38δ. Thus, even an ORF2-mutated L1 element, as expressed in RA, is biologically active and both L1 ORF1 and p38δ transcripts may appear as a consequence of genomic hypomethylation. The induction of p38δ appears to be mediated by an ORF1/p40-dependent process. This is the first indication of a p40 mediated transactivation.