Antioxidant Activities In Vitro of Ethanol Extract and Fractions from Mushroom, Lenzites Betulina
The antioxidant potential of different fractions of Lenzites betulina was investigated. Fraction 2 showed the highest antioxidant activity in the 2, 2‐diphenyl‐1‐picrylhydrazyl (DPPH) and hydroxyl (OH) radical‐scavenging assays. Three natural p‐terphenyls were identified in this fraction. The struct...
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Published in | Journal of food biochemistry Vol. 37; no. 6; pp. 687 - 693 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Blackwell Publishing Ltd
01.12.2013
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Online Access | Get full text |
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Summary: | The antioxidant potential of different fractions of Lenzites betulina was investigated. Fraction 2 showed the highest antioxidant activity in the 2, 2‐diphenyl‐1‐picrylhydrazyl (DPPH) and hydroxyl (OH) radical‐scavenging assays. Three natural p‐terphenyls were identified in this fraction. The structure of a new natural compound was established as 2‐phenyl‐[1H‐2‐benzopyran][4,3‐e][p]benzoquinone in addition to two other compounds, 2,5‐diphenyl‐3,6‐dimethoxy‐p‐benzoquinone and 2‐phenyl‐3‐methoxy‐[1H‐2‐benzopyran][4,3‐e][p]benzoquinone that have been previously reported in L. betulina. The antioxidant activities of these p‐terphenyls were evaluated by DPPH and OH radical‐scavenging assays. 2‐phenyl‐3‐methoxy‐[1H‐2‐benzopyran][4,3‐e][p]benzoquinone was the most active in the OH radical‐scavenging test (IC
50 = 37.69 μg/mL) and showed little inhibition in the DPPH assay. The chemical components of L. betulina therefore have some antioxidant capacities, and this species could be used as a potential source of new natural antioxidants.
Practical Applications
Lenzites betulina has been used as a kind of medicinal fungus in China. In order to find its potential application, the antioxidant potential of L. betulina was investigated. It was found that the total ethanol extracts and the fraction 2 of L. betulina showed strong antioxidative capacity in the 2, 2‐diphenyl‐1‐picrylhydrazyl and hydroxyl (OH) radical‐scavenging assays. The OH radical‐scavenging activity of the fraction 2 (IC
50 = 19.42 ± 0.4 μg/mL) was similar to that of ascorbic acid (IC
50 = 20.55 ± 0.2 μg/mL). Meanwhile, three natural p‐terphenyl (a new natural compound and two known compounds) were identified in this fraction. Compound 2 showed the most activity in the OH radical‐scavenging test (IC
50 = 37.69 μg/mL), so this species could be used as a potential source of new natural antioxidants. |
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Bibliography: | "111" Project - No. B08044 "985" Project - No. MUC985 National Basic Research Program of China - No. 973 Program, 2009CB522300 ArticleID:JFBC666 Program of State Ethnic Affairs Commission - No. 09ZY09 istex:AABC66B3B553C173C8E5D8CAC25B8F9FCCBA3665 The work was carried out at Minzu University of China, Beijing, China. Fundamental Research Funds for the Central Universities - No. 2009kyqnyjs22, 0910KYZY46 ark:/67375/WNG-W8X2MBGV-4 Fund of State Key Laboratory of Phytochemistry and Plant Resources in West China - No. 09708211Z1 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0145-8884 1745-4514 |
DOI: | 10.1111/j.1745-4514.2012.00666.x |