Cell-mediated cytotoxicity toward canine kidney epithelial cells

Cellular cytotoxicity toward kidney cell targets has been studied in a model using in vitro cultured canine kidney cells obtained after perfusion trypsinization of kidneys from one-haplotype-mismatched beagles. To study whether cytotoxic effector cells recognize identical antigens on kidney cells as...

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Bibliographic Details
Published inTransplantation Vol. 33; no. 5; p. 465
Main Authors Vegt, P A, Buurman, W A, van der Linden, C J, Daemen, A J, Greep, J M, Jeekel, J
Format Journal Article
LanguageEnglish
Published United States 01.05.1982
Subjects
Animals
Binding, Competitive
Cytotoxicity, Immunologic
Dogs
Epithelial Cells
Epithelium - immunology
Immunity, Cellular
Immunosorbent Techniques
Kidney - cytology
Kidney - immunology
Lymphocyte Activation
Phytohemagglutinins - pharmacology
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Summary:Cellular cytotoxicity toward kidney cell targets has been studied in a model using in vitro cultured canine kidney cells obtained after perfusion trypsinization of kidneys from one-haplotype-mismatched beagles. To study whether cytotoxic effector cells recognize identical antigens on kidney cells as on phytohemagglutinin (PHA)-stimulated lymphoblasts, adsorption studies with different monolayers have been performed. Both leukocyte and kidney cell monolayers reduced cytotoxicity against 51Cr-labeled PHA-stimulated lymphoblasts very effectively. The average reduction of cytotoxicity was 86% in six consecutive experiments after one adsorption on either one of these two types of target-specific monolayers. Nonspecific monolayers reduced cytotoxicity only for 13%. Specific kidney cell monolayers reduced cytotoxicity against kidney cells almost completely, however leukocyte monolayers reduced cytotoxicity toward kidney cells for only 40%. There results and cold target inhibition data strongly suggest that kidney cells present antigens to which a selective population of cytotoxic T lymphocytes (CTLs) is directed. These CTLs are not cytotoxic for PHA-stimulated lymphoblasts. It is discussed whether the relevant antigens on the kidney cells are organ-specific antigens comparable to the endothelial monocyte antigen system as described by Moreas and Stastny or that class II antigens are involved in cytotoxicity toward kidney cells.
ISSN:0041-1337
1534-6080
DOI:10.1097/00007890-198205000-00002