Glucose biofuel cells using the two-step reduction reaction of bienzyme structure as cathodic catalyst

• Published in: Journal of industrial and engineering chemistry (Seoul, Korea) Vol. 71; pp. 435 - 444
• Main Authors: Christwardana, Marcelinus, Chung, Yongjin, Kim, Do-Heyoung, Kwon, Yongchai
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 25.03.2019; 한국공업화학회
• Subjects: Bienzyme; Biofuel cell; Glucose oxidase; Horseradish peroxidase; π–π stacking; 화학공학; Horseradish peroxidase; Glucose oxidase; Bienzyme; π–π stacking; Biofuel cell
• ISSN: 1226-086X; 1876-794X
• DOI: 10.1016/j.jiec.2018.11.056

[Display omitted] •We suggest CNT/PBA/[HRP/PEI/GOx] catalyst as cathodic catalyst of GBFC.•Bienzyme structure consisting of GOx and HRP are used as the catalyst.•ORR is improved because of bienzyme entrapped well in PEI.•CNT/PBA promotes electron transfer due to π–π stacking and short pathway distance.•GBFC containing CNT/PBA/[HRP/PEI/GOx] catalyst induces high performance. Glucose oxidase (GOx) and horseradish peroxidase (HRP) based bienzymes are entrapped in a polyethylenimine (PEI) matrix and then immobilized onto a substrate consisting of carbon nanotube (CNT) and pyrene boronic acid (PBA) (CNT/PBA/[HRP/PEI/GOx]). This structure is considered as the cathodic catalyst for the glucose biofuel cell (GBFC). According to the performance evaluations of the catalyst, the catalytic activity for the oxygen reduction reaction (ORR) is improved because of the bienzyme entrapped well in PEI, and also, because the CNT/PBA substrate promotes electron transfer by the formation of π–π stacking and the reduction of the electron transfer pathway distance. The high ORR reaction rate (38.7μAcm−2 in the injection of 5mM glucose) is clear evidence of an excellent catalyst. In addition, when the catalyst is adopted for the operation of a membrane GBFC, a high-power density (77.9±2.3μWcm−2) is achieved, as well as good storage stability (81% of its initial activity even after 4 weeks) and a high glucose consumption rate (8.7±0.2% from its initial concentration during 7 operating hours). Alternatively, when the catalyst is used for the membraneless GBFC operation, a relatively high-power density of 18μWcm−2 is also obtained.