Activatable probes for non-invasive small animal fluorescence imaging
Fluorescence tomography today appears as a complementary imaging modality for assessing molecular processes in small living animals. A new design of fluorescent activatable units for the imaging of cellular internalization of probes is proposed. Disulfide bridges are used as intracellular cleavable...
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Published in | Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment Vol. 571; no. 1; pp. 165 - 168 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
01.02.2007
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Fluorescence tomography today appears as a complementary imaging modality for assessing molecular processes in small living animals. A new design of fluorescent activatable units for the imaging of cellular internalization of probes is proposed. Disulfide bridges are used as intracellular cleavable bounds for fluorescence activation. These units are not activated in blood, neither in vitro nor in mouse, but specifically in the presence of lysed cells or a chemical reducer. We therefore expect that the use of such activatable units, grafted to targeting moieties, will improve significantly the contrast of images obtained in the future. |
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ISSN: | 0168-9002 1872-9576 |
DOI: | 10.1016/j.nima.2006.10.053 |