Activatable probes for non-invasive small animal fluorescence imaging

Fluorescence tomography today appears as a complementary imaging modality for assessing molecular processes in small living animals. A new design of fluorescent activatable units for the imaging of cellular internalization of probes is proposed. Disulfide bridges are used as intracellular cleavable...

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Published inNuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment Vol. 571; no. 1; pp. 165 - 168
Main Authors Texier, Isabelle, Razkin, Jésus, Josserand, Véronique, Boturyn, Didier, Dumy, Pascal, Coll, Jean-Luc, Rizo, Philippe
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.02.2007
Elsevier
Subjects
Activatable probes
Animal imaging
Fluorescent dyes
Optical imaging
Reporter probes
87.64.Ni
Reporter probes
33.50.Dq
Fluorescent dyes
Optical imaging
Activatable probes
Animal imaging
87.57.−s
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Summary:Fluorescence tomography today appears as a complementary imaging modality for assessing molecular processes in small living animals. A new design of fluorescent activatable units for the imaging of cellular internalization of probes is proposed. Disulfide bridges are used as intracellular cleavable bounds for fluorescence activation. These units are not activated in blood, neither in vitro nor in mouse, but specifically in the presence of lysed cells or a chemical reducer. We therefore expect that the use of such activatable units, grafted to targeting moieties, will improve significantly the contrast of images obtained in the future.
ISSN:0168-9002
1872-9576
DOI:10.1016/j.nima.2006.10.053