POLYMORPHISM IN SUGAR BEET VARIETIES AND HYBRIDS IN CELL SELECTION FOR RESISTANCE TO ABIOTIC FACTORS

In the article, the results on the development of cell selection scheme for sugar beet genotypes resistant to high temperature are presented. In terms of increase in the callus diameter and the ability to regenerate plants, lines of high-temperature resistant genotypes Yaltushkivskyi MS 72, Ukrainia...

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Bibliographic Details
Published inJournal of microbiology, biotechnology and food sciences Vol. 7; no. 6; pp. 602 - 606
Main Authors Klyachenko, Oksana, Prysiazhniuk, Larysa
Format Journal Article
LanguageEnglish
Published Nitra Faculty of Biotechnology and Food Sciences 01.06.2018
Subjects
Abiotic factors
Alleles
Breeding
Callus
Cluster analysis
Deoxyribonucleic acid
DNA
Gene polymorphism
Genotypes
High temperature
Hybrids
Markers
Molecular chains
Polymorphism
Proline
Random amplified polymorphic DNA
Resistance factors
Temperature effects
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Summary:In the article, the results on the development of cell selection scheme for sugar beet genotypes resistant to high temperature are presented. In terms of increase in the callus diameter and the ability to regenerate plants, lines of high-temperature resistant genotypes Yaltushkivskyi MS 72, Ukrainian MS 70, Ukrainian MS 72 and Katiusha found to be promising for breeding. Experiments confirmed that 5-7 times increased proline content is typical of the temperature resistant lines. We investigated the molecular and genetic polymorphism in sugar beet genotypes using RAPD and SSR analyses. Fourteen alleles were identified and analyzed by four RAPD markers and five alleles by GZM 086 marker. Cluster analysis using DNA markers shows that Ukrainian MS 72 and Ivanivskyi MS 33 are the most similar genotypes, and they are found in the same cluster. It was determined that the temperature resistant sugar beet genotypes Yaltushkivskyi MS 72, Ukrainian MS 70, Ukrainian MS 72 and Katiusha are genetically distant and therefore can be used to create heterotic hybrids.
ISSN:1338-5178
1338-5178
DOI:10.15414/jmbfs.2018.7.6.602-606