Trichinella spiralis-derived extracellular vesicles induce a protective immunity against larval challenge in mice

• Published in: Pathogens and disease Vol. 80; no. 1
• Main Authors: Ashour, Dalia S, Ibrahim, Fatma M Kh, Elshamy, Amira M, Zoghroban, Hager S
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 12.11.2022
• Subjects: Defence mechanisms; Disease control; Electron microscopy; Electrophoresis; Extracellular vesicles; Flow cytometry; Gels; Immune response; Immune system; Immunity; Immunization; Immunoglobulin G; Inflammation; Intestine; Larvae; Lymphocytes T; Muscles; Parasites; Transmission electron microscopy; Trichinella spiralis; Trichinellosis; Vaccines; Vesicles; SDS-PAGE; vaccine candidate; derived extracellular vesicles; beads-based flow cytometry; EVs characterization
• ISSN: 2049-632X; 2049-632X
• DOI: 10.1093/femspd/ftac040

Abstract Human trichinellosis is a serious disease with no effective treatment till now. Recently, the protective immunity induced by parasite-derived extracellular vesicles (EVs) are studied for some parasites such as Echinostoma caproni. The current study aimed to investigate the novel Trichinella spiralis-derived EVs as a potential vaccine candidate for the first time in a mouse model. Trichinella spiralis EVs were isolated and identified using transmission electron microscopy, gel electrophoresis, protein content measurements, and beads-based flow cytometry. Vaccination was done by subcutaneous injection of two doses of 3.5 μg T. spiralis-derived EVs. We observed a significant reduction in T. spiralis adult worm and muscle larval counts in mice immunized with T. spiralis-derived EVs (EVs-Ts group) and controlled inflammatory changes in the intestine and muscles. The EVs-Ts group showed a higher level of IFN- γ, whereas the IL-4 secretion was elevated more in the EVs group (EVs group) and showed a lower level after challenge with T. spiralis infection (EVs-Ts group). This implies a mixed Th1/Th2 immune response with obvious Th1 polarization. Moreover, elevation of serum T. spiralis-specific IgG was reported. In conclusion, this preliminary study provides T. spiralis EVs as a promising candidate for future development of anti-Trichinella vaccine. Human trichinellosis is a serious disease with no effective treatment or vaccination till now. Trichinella spiralis extracellular vesicles is a promising candidate for future development of anti-Trichinella vaccine.