Reverse phase thin-layer chromatographic separation of bromodiphenhydramine and diphenhydramine

A thin-layer chromatographic system is described for the separation of diphenhydramine and bromodiphenhydramine which is based on a reverse phase paper chromatographic system developed by J. Vecerková (2,3) in 1962. Chromatographic systems for this separation described in the literature and several...

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Bibliographic Details
Published inJournal of chromatographic science Vol. 13; no. 12; p. 563
Main Authors Fusari, S A, Terhalle, M, Holcomb, I J, Alix, E C
Format Journal Article
LanguageEnglish
Published United States 01.12.1975
Subjects
Chromatography, Thin Layer - methods
Diphenhydramine - analogs & derivatives
Diphenhydramine - analysis
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Summary:A thin-layer chromatographic system is described for the separation of diphenhydramine and bromodiphenhydramine which is based on a reverse phase paper chromatographic system developed by J. Vecerková (2,3) in 1962. Chromatographic systems for this separation described in the literature and several systems attempted in this laboratory using normal chromatography have not proven successful. The method using reverse phase thin-layer chromatography involves a stationary phase of mineral oil on silica gel G and a mobile phase of ethanol:water:ammonium hydroxide, 28% NH3, (55:43:2). A 10-cm length of run requires about three hours and provides an excellent separation of the two compounds for identification-differentiation purposes.
ISSN:0021-9665
DOI:10.1093/chromsci/13.12.563