Complete mitochondrial DNA sequence analysis of Bison bison and bison-cattle hybrids: function and phylogeny

• Published in: Mitochondrion Vol. 11; no. 1; pp. 166 - 175
• Main Authors: Douglas, Kory C, Halbert, Natalie D, Kolenda, Claire, Childers, Christopher, Hunter, David L, Derr, James N
• Format: Journal Article
• Language: English
• Published: Netherlands 01.01.2011
• Subjects: Animals; Base Sequence; Bison; Bison - classification; Bison - genetics; Bison bison; Cattle - classification; Cattle - genetics; DNA, Mitochondrial - chemistry; DNA, Mitochondrial - genetics; DNA, Mitochondrial - metabolism; Genetics, Population; Genome, Mitochondrial; Haplotypes; Hybridization, Genetic; Mitochondria - genetics; Molecular Sequence Data; Phylogeny; Sequence Analysis, DNA
• ISSN: 1567-7249; 1872-8278
• DOI: 10.1016/j.mito.2010.09.005

Complete mitochondrial DNA (mtDNA) genomes from 43 bison and bison-cattle hybrids were sequenced and compared with other bovids. Selected animals reflect the historical range and current taxonomic structure of bison. This study identified regions of potential nuclear-mitochondrial incompatibilities in hybrids, provided a complete mtDNA phylogenetic tree for this species, and uncovered evidence of bison population substructure. Seventeen bison haplotypes defined by 66 polymorphic sites were discovered, whereas 728 fixed differences and 86 non-synonymous mutations were identified between bison and bison-cattle hybrid sequences. The potential roles of the mtDNA genome in the function of hybrid animals and bison taxonomy are discussed.