Lateral flow biosensor for DNA extraction-free detection of salmonella based on aptamer mediated strand displacement amplification

• Published in: Biosensors & bioelectronics Vol. 56; pp. 192 - 197
• Main Authors: Fang, Zhiyuan, Wu, Wei, Lu, Xuewen, Zeng, Lingwen
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.06.2014; Elsevier
• Subjects: Aptamer; Aptamers, Nucleotide - chemistry; Base Sequence; Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensors; Biotechnology; diagnostic techniques; DNA; DNA, Bacterial - analysis; DNA, Bacterial - isolation & purification; Equipment Design; Food Microbiology; food pathogens; Fundamental and applied biological sciences. Psychology; Humans; Lateral flow biosensor; Methods. Procedures. Technologies; Molecular Sequence Data; Nucleic Acid Amplification Techniques - instrumentation; oligonucleotides; Point-of-care; Point-of-Care Systems; rapid methods; Salmonella; Salmonella Enteritidis; Salmonella enteritidis - isolation & purification; Salmonella Infections - microbiology; Sensitivity and Specificity; Various methods and equipments; Salmonella; Lateral flow biosensor; Aptamer; Point-of-care; Amplification; Biosensor; DNA; Bacteria; Extraction; Detection; Enterobacteriaceae
• ISSN: 0956-5663; 1873-4235; 1873-4235
• DOI: 10.1016/j.bios.2014.01.015

Convenient and sensitive point-of-care rapid diagnostic tests for food-borne pathogens have been a long-felt need of clinicians. Traditional approaches such as culture-based methods have good sensitivity and specificity, but they tend to be tedious and time-consuming. Herein we present a simple and sensitive aptamer based biosensor for rapid detection of Salmonella enteritidis (S. enteritidis). One of the aptamers specific for the outmembrane of S. enteritidis was used for magnetic bead enrichments. Another aptamer against S. enteritidis was used as a reporter for this pathogen, which was amplified by isothermal strand displacement amplification (SDA) and further detected by a lateral flow biosensor. As low as 101 colony forming unit (CFU) of S. enteritidis was detected in this study. Without DNA extraction, the reduced handling and simpler equipment requirement render this assay a simple and rapid alternative to conventional methods. •The gold-nanoparticle based lateral flow biosensor is extremely simple to use and enables visual detection.•The tedious cell lysis and DNA/RNA extraction was avoided, while a high sensitivity (101CFU/mL) was achieved.•The specificity of the present assay is relative higher than that of nucleic acid based method. It has better response to the viable pathogens in the sample.