Influence of fiber architecture and growth factor formulation on osteoblastic differentiation of mesenchymal stem cells in coacervate-coated electrospun fibrous scaffolds

• Published in: Journal of industrial and engineering chemistry (Seoul, Korea) Vol. 79; pp. 236 - 244
• Main Authors: Kim, Sungjun, Lee, Jinkyu, Hwang, Mintai P., Wang, Yadong, Kim, Kyobum
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 25.11.2019; 한국공업화학회
• Subjects: Bone morphogenetic protein 2; Coacervate; Fibrous scaffold; Osteogenic differentiation; Platelet-derived growth factor; Poly(L-lactic acid); 화학공학; Fibrous scaffold; Coacervate; Poly(L-lactic acid); Platelet-derived growth factor; Bone morphogenetic protein 2; Osteogenic differentiation
• ISSN: 1226-086X; 1876-794X
• DOI: 10.1016/j.jiec.2019.06.044

[Display omitted] In order to achieve effective bone regeneration, the architectural/structural and biological environment must be considered. In this study, we investigated the influence of structure of extracellular matrix-mimicking electrospun poly (l-lactic acid) (PLLA) fibrous scaffolds and incorporated growth factors (bone morphogenetic protein2 (BMP2) and platelet-derived growth factor (PDGF)) (GFs) on in vitro differentiation of human mesenchymal stem cells (hMSCs) cultured onto the scaffolds. Furthermore, Cargo GFs were first encapsulated into coacervate (Coa). Coa-coated nano-sized PLLA (Coa-nPLLA) exhibited relatively higher coating efficacy, than Coa-coated micro-sized PLLA (Coa-mPLLA) due to large mesh hole area of mPLLA. Consequently, a faster GF release pattern was observed in nPLLA groups. As a result of osteogenic differentiation of hMSC which cultured onto GFs loaded Coa-PLLA, the architectural cue of fiber diameters effectively modulated early osteogenic differentiation of hMSCs. Facilitated long-term differentiation of hMSCs could be achieved by synergistic effect of dual BMP2 and PDGF in nanofbrous environments.