Bi-functional quantum dot-polysaccharide-antibody immunoconjugates for bioimaging and killing brain cancer cells in vitro

• Published in: Materials letters Vol. 252; pp. 333 - 337
• Main Authors: Santana, Cleildo P., Mansur, Alexandra A.P., Carvalho, Sandhra M., da Silva-Cunha, Armando, Mansur, Herman S.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.10.2019; Elsevier BV
• Subjects: Aqueous solutions; Bioconjugates; Brain; Brain cancer; Cancer; Cancer nanotechnology; Chitosan; Fluorescence; Growth factors; Materials science; Medical imaging; Monoclonal antibodies; Nanoconjugates; Nanoparticles; Polysaccharide; Polysaccharides; Quantum dots; Receptors; Nanoparticles; Cancer nanotechnology; Chitosan; Bioconjugates; Polysaccharide; Nanoconjugates
• ISSN: 0167-577X; 1873-4979
• DOI: 10.1016/j.matlet.2019.06.022

Nanotheranostics – Smart nanohybrid structures for target cancer immunotherapy. [Display omitted] •New bi-functional quantum dot-polysaccharide-antibody immunoconjugates were produced.•Fluorescent Ag-In-S/ZnS-chitosan nanocolloids produced via facile green aqueous route.•QD-chitosan nanocolloids biofunctionalized by VEGF antibody targeted cancer cells.•Novel Ag-In-S/ZnS-chitosan immunoconjugates behaved as fluorescent bio-nanoprobes.•The immunoconjugates were effective for bioimaging and killing brain cancer cells. The most common primary brain tumors in adults are gliomas, where glioblastoma multiforme is one of the deadliest in humans. In this study, we engineered novel nanoconjugates composed of Ag-In-S/ZnS quantum dots directly stabilized by chitosan polysaccharide ligand in aqueous medium and biofunctionalized with monoclonal antibody against vascular endothelial growth factor (VEGF) receptors. These nano-immunoconjugates demonstrated bi-functional capabilities combining fluorescent emission for bioimaging and killing activity towards brain cancer cells with very promising perspectives in nanotheranostic applications.