In vitro Remineralizing Effect of Fluoride Varnishes Containing Sodium Trimetaphosphate

• Published in: Caries research Vol. 48; no. 4; pp. 299 - 305
• Main Authors: Manarelli, M.M., Delbem, A.C.B., Lima, T.M.T., Castilho, F.C.N., Pessan, J.P.
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland S. Karger AG 01.01.2014
• Subjects: Anatomy, Cross-Sectional; Animals; Biopsy; Calcium Fluoride - analysis; Cariostatic Agents - pharmacology; Cattle; Dental Enamel - chemistry; Dental Enamel - drug effects; Dentistry; Dose-Response Relationship, Drug; Drug Synergism; Fluorides, Topical - pharmacology; Fluorides, Topical - therapeutic use; Hardness; Hydrogen-Ion Concentration; Original Paper; Placebos; Polyphosphates - pharmacology; Sodium Fluoride - therapeutic use; Time Factors; Tooth Demineralization - physiopathology; Tooth Remineralization - methods; pH-cycling; Fluoride varnish; Polyphosphates; Remineralization
• ISSN: 0008-6568; 1421-976X
• DOI: 10.1159/000356308

This study analyzed the effects of fluoride varnishes supplemented with sodium trimetaphosphate (TMP) on the remineralization of caries-like lesions in vitro. Bovine enamel discs were selected through surface hardness (SH) and caries-like lesions were induced. SH was again determined and the blocks were divided into 7 experimental groups (n = 24/group): placebo (no fluoride or TMP), 5% TMP, 2.5% NaF, 2.5% NaF/5% TMP, 5% NaF, 5% NaF/5% TMP and commercial formulation (Duraphat™, 5% NaF), following a blind protocol. Discs were treated with the varnishes and kept in a remineralizing solution for 4 h and a demineralizing solution for 2 h. Varnishes were then removed and half of the discs were used for analysis of loosely (CaF 2 ) and firmly bound fluoride. The remaining discs were submitted to a pH-cycling regimen for 6 days. The percentage of surface hardness recovery (%SHR), cross-sectional hardness (ΔKHN) and enamel CaF 2 and fluoride were determined. Data were analyzed by ANOVA and by Student-Newman-Keuls' test (p < 0.05). A dose-response relationship was observed between fluoride concentrations in the varnishes and %SHR. The 5% TMP varnish led to %SHR similar to that obtained for the placebo. When TMP was used in association with fluoride, however, significantly higher %SHR were observed in comparison with their counterparts without TMP. Moreover, ΔKHN obtained for the 5% NaF/5% TMP was significantly lower among all groups tested. Higher concentrations of CaF 2 and fluoride were observed for Duraphat and 5% NaF, followed by 5% NaF/5% TMP, 2.5% NaF and 2.5% NaF/5% TMP (p < 0.05). It was concluded that the supplementation of fluoride varnishes with TMP leads to enhanced remineralizing effect of artificial caries lesions in vitro.