Suitability of cryopreserved samples for pharmacological studies of ciliary activity
The effect of terbutaline sulfate on the ciliary activity of fresh and cryopreserved human nasal epithelium was evaluated. Cryopreservation had no effect on baseline ciliary beat frequency. Both fresh and cryopreserved samples exposed to 10(-4) M terbutaline showed a statistically significant increa...
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| Published in | Cryobiology Vol. 27; no. 6; pp. 591 - 595 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
San Diego, CA
Elsevier
01.12.1990
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| Subjects | |
| Online Access | Get full text |
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| Abstract | The effect of terbutaline sulfate on the ciliary activity of fresh and cryopreserved human nasal epithelium was evaluated. Cryopreservation had no effect on baseline ciliary beat frequency. Both fresh and cryopreserved samples exposed to 10(-4) M terbutaline showed a statistically significant increase in ciliary beat frequency (27 and 25%, respectively). When the percentage changes after drug challenge for fresh and cryopreserved samples were compared no statistical difference emerged. It is concluded that cryopreservation in liquid nitrogen at -196 degrees C does not affect membrane receptors, at least beta-adrenergic receptors, and therefore cryopreserved samples are suitable for pharmacological studies of ciliary activity. |
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| AbstractList | The effect of terbutaline sulfate on the ciliary activity of fresh and cryopreserved human nasal epithelium was evaluated. Cryopreservation had no effect on baseline ciliary beat frequency. Both fresh and cryopreserved samples exposed to 10(-4) M terbutaline showed a statistically significant increase in ciliary beat frequency (27 and 25%, respectively). When the percentage changes after drug challenge for fresh and cryopreserved samples were compared no statistical difference emerged. It is concluded that cryopreservation in liquid nitrogen at -196 degrees C does not affect membrane receptors, at least beta-adrenergic receptors, and therefore cryopreserved samples are suitable for pharmacological studies of ciliary activity. |
| Author | DI BENEDETTO, G GILL, J LOPEZ-VIDRIERO, M. T CLARKE, S. W |
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| Cites_doi | 10.1152/ajpcell.1984.247.3.C125 10.1159/000193106 10.1016/0011-2240(89)90056-4 10.1099/00222615-6-1-123 10.1164/ajrccm/139.2.432 10.1038/196592a0 10.1016/S0140-6736(80)91995-9 10.1016/0011-2240(85)90164-6 |
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| Keywords | Human Methodology Respiratory epithelium Epithelium Respiratory system Biological activity β-Adrenergic receptor Respiratory tract Membrane receptor Cryopreservation Nose Liquid nitrogen Ciliary activity |
| Language | English |
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| References | Lopez-Vidriero (10.1016/S0011-2240(05)80026-4_bib6) 1981; 86 Clarke (10.1016/S0011-2240(05)80026-4_bib1) 1983; 336 Santa Cruz (10.1016/S0011-2240(05)80026-4_bib13) 1974; 109 Rutland (10.1016/S0011-2240(05)80026-4_bib11) 1980; 2 Van As (10.1016/S0011-2240(05)80026-4_bib15) 1974; 31 Wulffraat (10.1016/S0011-2240(05)80026-4_bib17) 1985; 22 Dalhamn (10.1016/S0011-2240(05)80026-4_bib2) 1962; 196 Mazur (10.1016/S0011-2240(05)80026-4_bib8) 1984; 247 Sanderson (10.1016/S0011-2240(05)80026-4_bib12) 1989; 139 Hesse (10.1016/S0011-2240(05)80026-4_bib5) 1981; 31 Sleigh (10.1016/S0011-2240(05)80026-4_bib14) 1977 Morris (10.1016/S0011-2240(05)80026-4_bib9) 1973; 6 Greenstone (10.1016/S0011-2240(05)80026-4_bib4) 1983; 38 Lopez-Vidriero (10.1016/S0011-2240(05)80026-4_bib7) 1982; 124 Di Benedetto (10.1016/S0011-2240(05)80026-4_bib3) 1989; 26 Wanner (10.1016/S0011-2240(05)80026-4_bib16) 1977; 116 Pavia (10.1016/S0011-2240(05)80026-4_bib10) 1980; 16 |
| References_xml | – volume: 16 start-page: 335 year: 1980 ident: 10.1016/S0011-2240(05)80026-4_bib10 article-title: Deposition and clearance of inhaled particles publication-title: Bull. Eur. Physiopathol. Respir. contributor: fullname: Pavia – volume: 247 start-page: C125 year: 1984 ident: 10.1016/S0011-2240(05)80026-4_bib8 article-title: Freezing of living cells: Mechanisms and implications publication-title: Amer. J. Physiol. doi: 10.1152/ajpcell.1984.247.3.C125 contributor: fullname: Mazur – volume: 31 start-page: 146 year: 1974 ident: 10.1016/S0011-2240(05)80026-4_bib15 article-title: The role of selective β2-adrenoceptor stimulants in the control of ciliary activity publication-title: Respiration doi: 10.1159/000193106 contributor: fullname: Van As – volume: 26 start-page: 328 year: 1989 ident: 10.1016/S0011-2240(05)80026-4_bib3 article-title: The effect of cryopreservation on ciliary beat frequency of human respiratory epithelium publication-title: Cryobiology doi: 10.1016/0011-2240(89)90056-4 contributor: fullname: Di Benedetto – volume: 109 start-page: 458 year: 1974 ident: 10.1016/S0011-2240(05)80026-4_bib13 article-title: Tracheal mucous velocity in normal man and patients with obstructive lung disease: Effects of terbutaline publication-title: Amer. Rev. Respir. Dis. contributor: fullname: Santa Cruz – volume: 86 start-page: 799 issue: 6 year: 1981 ident: 10.1016/S0011-2240(05)80026-4_bib6 article-title: Airways mucus: Production and composition publication-title: Chest contributor: fullname: Lopez-Vidriero – volume: 6 start-page: 123 year: 1973 ident: 10.1016/S0011-2240(05)80026-4_bib9 article-title: Preservation of human tracheal organ cultures at−196°C publication-title: Med. Microbiol. doi: 10.1099/00222615-6-1-123 contributor: fullname: Morris – volume: 124 start-page: 244 issue: 4 year: 1982 ident: 10.1016/S0011-2240(05)80026-4_bib7 article-title: Objective, criteria for measuring ciliary beat frequency in vitro publication-title: Amer. Rev. Respir. Dis. contributor: fullname: Lopez-Vidriero – volume: 139 start-page: 432 year: 1989 ident: 10.1016/S0011-2240(05)80026-4_bib12 article-title: Mechanosensitive and β-adrenergic control of the ciliary beat frequency of mammalian respiratory tract cells in culture publication-title: Amer. Rev. Respir. Dis. doi: 10.1164/ajrccm/139.2.432 contributor: fullname: Sanderson – volume: 116 start-page: 73 year: 1977 ident: 10.1016/S0011-2240(05)80026-4_bib16 article-title: Clinical aspects of mucociliary transport publication-title: Amer. Rev. Respir. Dis contributor: fullname: Wanner – volume: 336 start-page: 40 year: 1983 ident: 10.1016/S0011-2240(05)80026-4_bib1 article-title: The effect of a β2-agonist on the activity of human bronchial cilia in vitro publication-title: J Physiol contributor: fullname: Clarke – volume: 196 start-page: 592 year: 1962 ident: 10.1016/S0011-2240(05)80026-4_bib2 article-title: Frequency of ciliary beat measured with a photo-sensitive cell publication-title: Nature doi: 10.1038/196592a0 contributor: fullname: Dalhamn – volume: 31 start-page: 716 year: 1981 ident: 10.1016/S0011-2240(05)80026-4_bib5 article-title: Influence of reproterol on ciliary beat frequency of human bronchial epithelium in vitro publication-title: Arzneimemm Forsch. contributor: fullname: Hesse – volume: 2 start-page: 564 year: 1980 ident: 10.1016/S0011-2240(05)80026-4_bib11 article-title: Non-invasive sampling of nasal cilia for measurement of beat frequency and study of ultrastructure publication-title: Lancet doi: 10.1016/S0140-6736(80)91995-9 contributor: fullname: Rutland – volume: 22 start-page: 105 year: 1985 ident: 10.1016/S0011-2240(05)80026-4_bib17 article-title: Frequency and coordination of ciliary beat after cryopreservation of respiratory epithelium publication-title: Cryobiology doi: 10.1016/0011-2240(85)90164-6 contributor: fullname: Wulffraat – volume: 38 start-page: 227 year: 1983 ident: 10.1016/S0011-2240(05)80026-4_bib4 article-title: Failure of isoprenaline to stimulate human respiratory cilia in vitro publication-title: Thorax contributor: fullname: Greenstone – start-page: 247 year: 1977 ident: 10.1016/S0011-2240(05)80026-4_bib14 article-title: The nature and action of respiratory tract cilia contributor: fullname: Sleigh |
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| SubjectTerms | Adult Air breathing Biological and medical sciences Cryopreservation Epithelium - drug effects Epithelium - physiology Female Fundamental and applied biological sciences. Psychology Humans In Vitro Techniques Male Mucociliary Clearance - drug effects Mucociliary Clearance - physiology Nasal Mucosa - drug effects Nasal Mucosa - physiology Receptors, Adrenergic, beta - drug effects Receptors, Adrenergic, beta - physiology Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics Terbutaline - pharmacology Vertebrates: respiratory system |
| Title | Suitability of cryopreserved samples for pharmacological studies of ciliary activity |
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