Suitability of cryopreserved samples for pharmacological studies of ciliary activity

• Published in: Cryobiology Vol. 27; no. 6; pp. 591 - 595
• Main Authors: DI BENEDETTO, G, GILL, J, LOPEZ-VIDRIERO, M. T, CLARKE, S. W
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier 01.12.1990
• Subjects: Adult; Air breathing; Biological and medical sciences; Cryopreservation; Epithelium - drug effects; Epithelium - physiology; Female; Fundamental and applied biological sciences. Psychology; Humans; In Vitro Techniques; Male; Mucociliary Clearance - drug effects; Mucociliary Clearance - physiology; Nasal Mucosa - drug effects; Nasal Mucosa - physiology; Receptors, Adrenergic, beta - drug effects; Receptors, Adrenergic, beta - physiology; Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics; Terbutaline - pharmacology; Vertebrates: respiratory system; Human; Methodology; Respiratory epithelium; Epithelium; Respiratory system; Biological activity; β-Adrenergic receptor; Respiratory tract; Membrane receptor; Cryopreservation; Nose; Liquid nitrogen; Ciliary activity
• ISSN: 0011-2240; 1090-2392
• DOI: 10.1016/S0011-2240(05)80026-4

The effect of terbutaline sulfate on the ciliary activity of fresh and cryopreserved human nasal epithelium was evaluated. Cryopreservation had no effect on baseline ciliary beat frequency. Both fresh and cryopreserved samples exposed to 10(-4) M terbutaline showed a statistically significant increase in ciliary beat frequency (27 and 25%, respectively). When the percentage changes after drug challenge for fresh and cryopreserved samples were compared no statistical difference emerged. It is concluded that cryopreservation in liquid nitrogen at -196 degrees C does not affect membrane receptors, at least beta-adrenergic receptors, and therefore cryopreserved samples are suitable for pharmacological studies of ciliary activity.