Gold nanoparticle-based plasmonic probe for selective recognition of adenosine

• Published in: Sensors and actuators. B, Chemical Vol. 296; p. 126591
• Main Authors: Du, Jianjun, Ge, Haoying, Long, Saran, Sun, Wen, Fan, Jiangli, Peng, Xiaojun
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.10.2019
• Subjects: Adenosine detection; Colorimetric assay; Eyedropper function; Gold nanoparticle; Plasmonic probe; Colorimetric assay; Plasmonic probe; Adenosine detection; Eyedropper function; Gold nanoparticle
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2019.05.068

•A novel colorimetric probe, creatinine-Ag+/AuNPs, was constructed for detection of adenosine.•The detection time for adenosine was only 4min.•The detection limit (45nM) satisfied the assay request of adenosine in serum.•The RGB value was introduced for fast, convenient, and quantitative determination of adenosine. Adenosine, as an endogenous molecule in organisms, plays an essential role in biological processes. Here, a plasmonic probe, creatinine-Ag+/gold nanoparticle (AuNPs), is assembled for adenosine detection based on synergistic coordination on AuNPs. The A650 nm/520 nm values of AuNPs system change linearly with adenosine concentration over a range of 1.0–5.0 μM and the detection limits reached 45 nM. The adenosine detection is realized within 4 min. Furthermore, the quantitative detection of adenosine is realized by eyedropper (a function in Microsoft’s PowerPoint) for analyzing RGB value changes of colorimetric assay. Therefore, this sensor can provide accurate and rapid assay of adenosine in patients’ serum sample without complicated instrumentations.